Antifungal activity of β-lapachone against azole-resistant Candida spp. and its aspects upon biofilm formation

The purpose of this study was to assess the antifungal effect of β-lapachone (β-lap) on azole-resistant strains of spp. in both planktonic and biofilm form. The antifungal activity of β-lap was evaluated by broth microdilution, flow cytometry and the comet assay. The cell viability of the biofilms w...

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Bibliographic Details
Published in:Future microbiology 2020-11, Vol.15 (16), p.1543-1554
Main Authors: da Silva, Cecília R, S Campos, Rosana de, de A Neto, João B, Sampaio, Letícia S, do Nascimento, Francisca Bsa, do Av Sá, Lívia G, Cândido, Thiago M, Magalhães, Hemerson If, da Cruz, Eduardo Hg, da Silva Júnior, Eufrânio N, de Moraes, Manoel O, Cavalcanti, Bruno C, Silva, Jacilene, Marinho, Emmanuel S, Júnior, Hélio Vn
Format: Article
Language:English
Subjects:
Antifungal activity
Antifungal agents
Biofilms
Candida
Candidiasis
Cell growth
Cell viability
Comet assay
Deoxyribonucleic acid
DNA
DNA damage
Drugs
Experiments
Flow cytometry
Infections
Laboratories
Lapachone
Metabolites
Natural products
Yeast
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Summary:The purpose of this study was to assess the antifungal effect of β-lapachone (β-lap) on azole-resistant strains of spp. in both planktonic and biofilm form. The antifungal activity of β-lap was evaluated by broth microdilution, flow cytometry and the comet assay. The cell viability of the biofilms was assessed using the MTT assay. β-lap showed antifungal activity against resistant strains of spp. in planktonic form. In addition, β-lap decreased the viability of mature biofilms and inhibited the formation of biofilms . β-lap showed antifungal activity against spp., suggesting that the compound can be utilized as an adjunct agent in the treatment of candidiasis.
ISSN:1746-0913
1746-0921
DOI:10.2217/fmb-2020-0011