Effect of different muscle contraction mode on the expression of Myostatin, IGF-1, and PGC-1 alpha family members in human Vastus Lateralis muscle

• Published in: Molecular biology reports 2020-12, Vol.47 (12), p.9251-9258
• Main Authors: Taghibeikzadehbadr, Pejman, Shirian, Sadegh, Sabouri, Mostafa
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Biopsy; Histology; Insulin-like growth factor I; Life Sciences; Morphology; mRNA; Muscle contraction; Myostatin; Original Article; Skeletal muscle
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-020-06017-x

Muscle contraction stimulates a transient change of myogenic factors, partly related to the mode of contractions. Here, we assessed the response of IGF - 1Ea , IGF - 1Eb, IGF - 1Ec, PGC1α -1, PGC1α -4, and myostatin to the eccentric Vs. the concentric contraction in human skeletal muscle. Ten healthy males were performed an acute eccentric and concentric exercise bout ( n  = 5 per group). For each contraction type, participants performed 12 sets of 10 repetitions knee extension by the dominant leg. Baseline and post-exercise muscle biopsy were taken 4 weeks before and immediately after experimental sessions from Vastus Lateralis muscle. Genes expression was measured by real-time PCR technique. There was a significant increase in PGC1α -1, PGC1α -4, IGF - 1Ea and, IGF - 1Eb mRNA after concentric contraction ( p  ≤ 0.05), while the PGC1α -4 and IGF - 1Ec significantly increased after eccentric contraction ( p  ≤ 0.05). It is intriguing to highlight that; no significant differences between groups were evident for changes in any variables following exercise bouts ( p  ≥ 0.05). Our results found that concentric and eccentric contractions presented different responses in PGC1α -1, IGF - 1Ea , IGF - 1Eb, and IGF - 1Ec mRNA. However, a similar significant increase in mRNA content was observed in PGC1α -4. Further, no apparent differences could be found between the response of genes to eccentric and concentric contraction.