spn-A/rad51 mutant exhibits enhanced genomic damage, cell death and low temperature sensitivity in somatic tissues

Homologous recombination (HR) is one of the key pathways to repair double-strand breaks (DSBs). Rad51 serves an important function of catalysing strand exchange between two homologous sequences in the HR pathway. In higher organisms, rad51 function is indispensable with its absence leading to early...

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Bibliographic Details
Published in:Chromosoma 2021-03, Vol.130 (1), p.3-14
Main Authors: Khan, Chaitali, Muliyil, Sonia, Ayyub, Champakali, Rao, B. J.
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biochemistry
Biomedical and Life Sciences
Bristles
Cell Biology
Cell death
Complementation
Developmental Biology
Developmental stages
Drosophila
Embryos
Eukaryotic Microbiology
Homologous recombination
Human Genetics
Insects
Lethality
Life Sciences
Low temperature
Mutants
Original Article
Pattern formation
Phenotypes
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Summary:Homologous recombination (HR) is one of the key pathways to repair double-strand breaks (DSBs). Rad51 serves an important function of catalysing strand exchange between two homologous sequences in the HR pathway. In higher organisms, rad51 function is indispensable with its absence leading to early embryonic lethality, thus precluding any mechanistic probing of the system. In contrast, the absence of Drosophila rad51 ( spn-A/rad51 ) has been associated with defects in the germline, without any reported detrimental consequences to Drosophila somatic tissues. In this study, we have performed a systematic analysis of developmental defects in somatic tissues of spn-A mutant flies by using genetic complementation between multiple spn-A alleles. Our current study, for the first time, uncovers a requirement for spn-A in somatic tissue maintenance during both larval and pupal stages. Also, we show that spn-A mutant exhibits patterning defects in abdominal cuticle in the stripes and bristles, while there appear to be only subtle defects in the adult wing and eye. Interestingly, spn-A mutant shows a discernible phenotype of low temperature sensitivity, suggesting a role of spn-A in temperature sensitive cellular processes. In summary, our study describes the important role played by spn-A/rad51 in Drosophila somatic tissues.
ISSN:0009-5915
1432-0886
DOI:10.1007/s00412-020-00746-5