Genetic analysis of anther culture response and identification of QTLs associated with response traits in wheat (Triticum aestivum L.)

Anther culture is the most effective tool for doubled haploid production of wheat. This investigation was conducted to estimate genetic parameters of anther culture response in wheat and identification of putative Quantitative trait loci (QTLs) associated with response traits. Two varieties of wheat...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biology reports 2020-12, Vol.47 (12), p.9289-9300
Main Authors: Abd El-Fatah, Bahaa E. S., Sayed, Mohammed A., El-Sanusy, Sahar A.
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
Callus
Gene mapping
Gene polymorphism
Genetic analysis
Histology
Life Sciences
Marker-assisted selection
Morphology
Original Article
Plant breeding
Polymorphism
Quantitative trait loci
Triticum aestivum
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Anther culture is the most effective tool for doubled haploid production of wheat. This investigation was conducted to estimate genetic parameters of anther culture response in wheat and identification of putative Quantitative trait loci (QTLs) associated with response traits. Two varieties of wheat, namely ICR-DH (P 1 ) and Sle 1 × 15 (P 2 ) and their F 1 and F 2 progenies were used in the present investigation to estimate genetic parameters of anther culture response. Two molecular marker systems, SRAP and SSR markers were used to detect the polymorphism between two anther donor parents. Single marker analysis (SMA) and Composite interval mapping (CIM) were used to localize the putative QTL associated with four anther culture response in wheat using 100 plants of F 2 population derived from F 1 cross ‘ICR-DH’ ×  ‘Sel 1 × 15’. Analyses of variance indicated significant differences between four populations (P 1 , P 2 , F 1 and F 2 ) for callus induction (CAL), number of green plants per 100 anther (GR), number of albino plant per 100 anther (AR) and total regenerated plants per 100 anther (TR). The additive effects were more important than dominance effects in controlling these traits. The two molecular marker systems were sufficient in detecting polymorphism between two parents. Thirty two putative QTLs were detected on eight linkage groups. Our study indicated that the additive effects of genes and detection of new QTLs permit marker-assisted selection of genotypes with high green plantlet regeneration efficiency in anther culture, and therefore favor efficient use of anther culture in wheat breeding programs
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-020-06007-z