Vitamin D supplementation improves bone mineralisation independent of dietary phosphate in male X-linked hypophosphatemic (Hyp) mice

• Published in: Bone (New York, N.Y.) N.Y.), 2021-02, Vol.143, p.115767-115767, Article 115767
• Main Authors: Barratt, Kate R., Sawyer, Rebecca K., Atkins, Gerald J., St-Arnaud, Rene, Anderson, Paul H.
• Format: Article
• Language: English
• Subjects: Fibroblast growth factor 23; Hyp; Rickets; Vitamin D; XLH
• ISSN: 8756-3282; 1873-2763
• DOI: 10.1016/j.bone.2020.115767

The disorder of X-linked hypophosphatemia (XLH), results in the supressed renal production of active 1α,25-dihydroxyvitamin D (1,25(OH)2D) due to elevated fibroblast growth factor-23 (FGF23) levels. While adequate 25(OH)D levels are generally associated with improved mineralisation of the skeleton independent of circulating 1,25(OH)2D levels, it is unclear whether raising 25(OH)D to sufficiently high levels through dietary vitamin D3 administration contributes to improving bone mineralisation in the murine homolog for XLH, Hyp mice. Three-week-old male Hyp mice were fed one of four diets containing either 1000 IU (C) or 20,000 IU (D) vitamin D3/kg diet with either 0.35% phosphate or 1.25% phosphate (P) until 12 weeks of age (n = 12/group). When compared to C-fed mice, D-fed mice significantly elevated serum 25(OH)D levels to 72.8 ± 4.9 nmol/L (2-fold, p