Coelomocyte replenishment in adult Asterias rubens: the possible ways

• Published in: Cell and tissue research 2021-03, Vol.383 (3), p.1043-1060
• Main Authors: Sharlaimova, Natalia, Shabelnikov, Sergey, Bobkov, Dan, Martynova, Marina, Bystrova, Olga, Petukhova, Olga
• Format: Article
• Language: English
• Subjects: Asterias rubens; Biomedical and Life Sciences; Biomedicine; Cell migration; Coelomocytes; Electron microscopy; Epithelial cells; Epithelium; Human Genetics; Molecular Medicine; Physiology; Proteomics; Regular Article; Regulatory proteins
• ISSN: 0302-766X; 1432-0878
• DOI: 10.1007/s00441-020-03337-z

The origin of cells involved in regeneration in echinoderms remains an open question. Replenishment of circulatory coelomocytes—cells of the coelomic cavity in starfish—is an example of physiological regeneration. The coelomic epithelium is considered to be the main source of coelomocytes, but many details of this process remain unclear. This study examined the role of coelomocytes outside circulation, named marginal coelomocytes and small undifferentiated cells of the coelomic epithelium in coelomocyte replenishment in Asterias rubens . A qualitative and quantitative comparison of circulatory and marginal coelomocytes, as well as changes of circulatory coelomocyte concentrations in response to injury at different physiological statuses, was analysed. The presence of cells morphologically similar to coelomocytes in the context of coelomic epithelium was evaluated by electron microscopy. The irregular distribution of small cells on the surface and within the coelomic epithelium was demonstrated and the origin of small undifferentiated cells and large agranulocytes from the coelomic epithelium was suggested. Two events have been proposed to mediate the replenishment of coelomocytes in the coelom: migration of mature coelomocytes of the marginal cell pool and migration of small undifferentiated cells of the coelomic epithelium. The proteomic analysis of circulatory coelomocytes, coelomic epithelial cells and a subpopulation of coelomic epithelial cells, enriched in small undifferentiated cells, revealed proteins that were common and specific for each cell pool. Among these molecules were regulatory proteins, potential participants of regenerative processes.