MicroRNA‐223 triggers inflammation in porcine aorta by activating NLRP3 inflammasome under selenium deficiency

Selenium (Se) is an essential trace element in organism. Se deficiency can cause many diseases, including vascular disease. Studies have shown that inflammation is the main inducement of vascular disease, microRNA (miRNA) can influence inflammation in various ways, and Se deficiency can affect miRNA...

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Bibliographic Details
Published in:Journal of cellular physiology 2021-06, Vol.236 (6), p.4555-4564
Main Authors: Qing, Zhang, Kaixin, Zhang, Yanfei, Han, Yiming, Zhang, Hua, Xue, Ling, Zhou, Guangliang, Shi, Shu, Li
Format: Article
Language:English
Subjects:
Aorta
Apoptosis
Arteries
Caspase
Cell lines
Coronary vessels
Endothelium
Inflammasomes
Inflammation
MicroRNAs
microRNA‐223
miRNA
mRNA
NLRP3
Nucleotides
Oligomerization
porcine
Proteins
Ribonucleic acid
RNA
Selenium
selenium deficient
Trace elements
Vascular diseases
vascular injury
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Summary:Selenium (Se) is an essential trace element in organism. Se deficiency can cause many diseases, including vascular disease. Studies have shown that inflammation is the main inducement of vascular disease, microRNA (miRNA) can influence inflammation in various ways, and Se deficiency can affect miRNAs expression. To study the mechanism of aorta damage caused by Se deficiency, we constructed a Se deficiency porcine aorta model and found that Se deficiency can significantly inhibit miR‐223, which downregulates the expression of nucleotide‐binding oligomerization domain‐like receptor family 3 (NLRP3). Subsequently, we found that in Se deficiency group, NLRP3, and its downstream (caspase‐1, apoptosis‐related spot‐like protein [ASC], IL‐18, IL‐1β) expression was significantly increased. In vitro, we cultured pig iliac endothelium cell lines, and constructed miR‐223 knockdown and overexpression models. NLRP3 messenger RNA and protein levels were significant increased in the knockdown group, and decreased in the overexpression group. The results of this study show that Se deficiency in porcine arteries can induce inflammation through miR‐223/NLRP3. (1) Se deficiency can cause inflammation of the aorta in pigs. (2) There is a targeting relationship between miR‐22 and Nlrp3. (3) Se deficiency can regulate Nlrp3 inflammasome in pig aorta through miR‐223.
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.30178