High‐Preservation Single‐Cell Operation through a Photo‐responsive Hydrogel‐Nanopipette System

Single‐cell and in situ cell‐based operation with nanopipette approach offers a possibility to elucidate the intracellular processes and may aid the improvement of therapy efficiency and precision. We present here a photo‐responsive hydrogel‐nanopipette hybrid system that can achieve single‐cell ope...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2021-03, Vol.60 (10), p.5157-5161
Main Authors: Li, Zi‐Yuan, Liu, Ying‐Ya, Li, Yuan‐Jie, Wang, Wenhui, Song, Yanyan, Zhang, Junji, Tian, He
Format: Article
Language:English
Subjects:
Cell lines
Cell survival
Cell viability
Damage
diarylethene
Dosage
Doxorubicin
Drug delivery
Electric potential
Hybrid systems
Hydrogels
Injection
potential-free nanopipette
precise dosing
single-cell operation
supramolecular hydrogel
Survival
Temporal resolution
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Summary:Single‐cell and in situ cell‐based operation with nanopipette approach offers a possibility to elucidate the intracellular processes and may aid the improvement of therapy efficiency and precision. We present here a photo‐responsive hydrogel‐nanopipette hybrid system that can achieve single‐cell operation with high spatial/temporal resolution and negligible cell damage. This strategy overcomes long‐time obstacles in nanopipette single‐cell studies as high electric potential (ca. 1000 mV) or organic solvent is always used during operations, which would inevitably impose disturbance and damage to targeted cells. The light‐triggered system promotes a potential‐free, non‐invasive single‐cell injection, resulting in a well‐retained cell viability (90 % survival rate). Moreover, the photo‐driven injection enables a precisely dose‐controllable single‐cell drug delivery. Significantly reduced lethal doses of doxorubicin (163–217 fg cell−1) are demonstrated in corresponding cell lines. The fabrication of photo‐responsive hydrogel‐nanopipette system ensures both precision single‐cell operation and high cell preservation. Upon light‐controlled, non‐invasive operation, a high cell viability over 90 % as well as precise quantification of injection are obtained. Hence, a single‐cell precise‐dosing is achieved with a minimum lethal dose of 163–217 fg cell−1.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.202013011