Long-Term Longitudinal Stability of Kidney Filtration Marker Measurements: Implications for Epidemiological Studies and Clinical Care

Abstract Background Establishment and improvement of glomerular filtration rate estimating equations requires accurate and precise laboratory measurement procedures (MPs) for filtration markers. The Advanced Research and Diagnostic Laboratory (ARDL) at the University of Minnesota, which has served a...

Full description

Saved in:
Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2021-02, Vol.67 (2), p.425-433
Main Authors: Karger, Amy B, Eckfeldt, John H, Rynders, Gregory P, Chaudhari, Juhi, Miao, Shiyuan, Van Lente, Frederick, Coresh, Josef, Levey, Andrew S, Inker, Lesley A
Format: Article
Language:English
Subjects:
Analysis
Calibration
Creatinine
Cystatin C
Epidemiology
Filtration
Glomerular filtration rate
Kidney diseases
Kidney function tests
Kidneys
Laboratories
Longitudinal stability
Markers
Methods
Monitoring
Proteins
Quality assurance
Quality control
Stability analysis
Telemedicine
Trends
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Background Establishment and improvement of glomerular filtration rate estimating equations requires accurate and precise laboratory measurement procedures (MPs) for filtration markers. The Advanced Research and Diagnostic Laboratory (ARDL) at the University of Minnesota, which has served as the central laboratory for the Chronic Kidney Disease Epidemiology Collaboration since 2009, has implemented several quality assurance measures to monitor the accuracy and stability of filtration marker assays over time. Methods To assess longitudinal stability for filtration marker assays, a 40-sample calibration panel was created using pooled serum, divided into multiple frozen aliquots stored at −80 °C. ARDL monitored 4 markers—creatinine, cystatin C, beta-2-microglobulin (B2M) and beta-trace protein—measuring 15 calibration panel aliquots from 2009 to 2019. Initial target values were established using the mean of the first 3 measurements performed in 2009–10, and differences from target were monitored over time. New MPs for cystatin C and B2M were added in 2012, with target values established using the first measurement. Results The mean percentage difference from mean target values across time was
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/hvaa237