Gene expression profile involved in signaling and apoptosis of osteoblasts in contact with cellulose/MWCNTs scaffolds

The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs. Osteoblasts were cultivated on BCN, MWCNTs and their mixt...

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Published in:Materials Science & Engineering C 2021-01, Vol.118, p.111531-111531, Article 111531
Main Authors: Gutiérrez-Hernández, José Manuel, Castorena-Alejandro, Claudia, Pozos-Guillén, Amaury, Toriz-González, Guillermo, Flores, Héctor, Escobar-García, Diana María
Format: Article
Language:English
Subjects:
Apoptosis
Bacterial cellulose nanofibers (BCN), multi-walled carbon nanotubes (MWCNTs), osteoblast
Cell proliferation
Cellulose
Nanotubes, Carbon
Osteoblasts
Transcriptome
Vasculogenesis
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Summary:The aim of this work was to evaluate the expression profile of genes involved in signaling, intracellular and extracellular Ca+2 concentration and apoptosis pathways of osteoblasts in contact with a scaffold made of a composite of BCN/MWCNTs. Osteoblasts were cultivated on BCN, MWCNTs and their mixtures. Osteoblast RNA was extracted for sintering cDNA to amplify genes of interest by PCR; intra- and extracellular calcium (Ca2+) was also quantified. Regarding the genes that participate in the regulation paths (MAPK and NF-KB), it was found that only the expression of NF-KB was affected in all treatments. The expression of VEGFA increased, except in the treatment of high concentration of MWCNTs, where remained unchanged. The expression of genes Apaf-1 and Bcl-2/Bax and TP53 increased as compared to the control (except for TP53 in BC and C1/MWCNTs) indicating that cells are responding to the presence of BCN-MWCNTs composites scaffolds. The results suggest that osteoblast developed a modification in the expression profile of genes that actively participate in cellular processes such as proliferation, vasculogenesis and apoptosis, which may be modulated by the increase of intra- and extracellular Ca2+ concentration. •Understanding of the effect of nanoestructures in the gene expression regulation involved in basic cellular processes•Gene expression profile involved in signaling and apoptosis cells contact with scaffolds•The gene expression involved in extracellular Ca+2 and apoptosis pathways of osteoblasts in contact with a BCN/MWCNT scaffold•The expression of transcription factor NF-KB in osteoblasts in contact with MWCNTs
ISSN:0928-4931
1873-0191
DOI:10.1016/j.msec.2020.111531