A bifunctionalized NiCo2O4-Au composite: Intrinsic peroxidase and oxidase catalytic activities for killing bacteria and disinfecting wound

• Published in: Journal of hazardous materials 2021-01, Vol.402, p.123939-123939, Article 123939
• Main Authors: Mu, Qianzhu, Sun, Yanfeng, Guo, Aiying, Xu, Xiaoyue, Qin, Baoping, Cai, Aijun
• Format: Article
• Language: English
• Subjects: Antibacterial; Catalysis; Nanozyme; NiCo2O4-Au; ROS
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2020.123939

[Display omitted] •A recyclable NiCo2O4-Au composite was fabricated.•The integration of Au with NiCo2O4 provided peroxidase- and oxidase-like activities.•NiCo2O4-Au could effectively kill bacteria and destruct biofilms with 100 μM H2O2.•NiCo2O4-Au based Band-Aid exhibited high anti-infective and wound-healing efficiency.•The antibacterial mechanism was the formation of OH and 1O2. A NiCo2O4-Au composite was fabricated following the calcination-reduction method and testified to exhibit the intrinsic high peroxidase- and oxidase-like activities. The composite could activate a low level of H2O2 (100 μM) to kill E. coli and S. aureus. NiCo2O4-Au composite could be easily separated by an external magnet from the media and reused several times. NiCo2O4-Au composite could also effectively damage the existing biofilms and prevent the formation of new biofilms. The electron spin resonance tests showed that NiCo2O4-Au composite catalyzed H2O2 into reactive oxygen species (ROS), mainly including OH, O2−, and 1O2; while the oxidase-like activity of NiCo2O4-Au also stemmed from the ROS formation in the absence of H2O2. The radical trapping experiment confirmed that OH and 1O2 were the main radicals in the antibacterial process for NiCo2O4-Au in the presence of H2O2. A NiCo2O4-Au based Band-Aid was also designed, which exhibited high anti-infective and wound-healing properties. This study has demonstrated that NiCo2O4-Au composite can be a promising antibacterial agent in environmental and clinical applications.