In Vitro Effects of Lithium Carbonate on Cell Cycle, Apoptosis, and Autophagy in Hepatocellular Carcinoma-29 Cells

We studied the effects of lithium carbonate on the cell cycle, apoptosis, and autophagy in hepatocellular carcinoma-29 cells (HCC-29) in vitro . Flow cytofluorometry analysis revealed accumulation of G2/M-phase HCC-29 cells and increase in the number of apoptotic cells in 48 h after administration o...

Full description

Saved in:
Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2020-12, Vol.170 (2), p.246-250
Main Authors: Taskaeva, Y. S., Bgatova, N. P., Dossymbekova, R. S., Solovieva, A. O., Miroshnichenko, S. M., Sharipov, K. O., Tungushbaeva, Z. B.
Format: Article
Language:English
Subjects:
Antitumor activity
Apoptosis
Autophagy
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell cycle
Cytofluorometry
Drug therapy
Flow cytometry
Hepatocellular carcinoma
Hepatoma
Immunofluorescence
Internal Medicine
Laboratory Medicine
Lithium
Lithium carbonate
Liver cancer
Pathology
Phagocytosis
Transmission electron microscopy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We studied the effects of lithium carbonate on the cell cycle, apoptosis, and autophagy in hepatocellular carcinoma-29 cells (HCC-29) in vitro . Flow cytofluorometry analysis revealed accumulation of G2/M-phase HCC-29 cells and increase in the number of apoptotic cells in 48 h after administration of 5 mM lithium carbonate. Induction of autophagy in HCC-29 cells was detected by transmission electron microscopy and immunofluorescence staining. Thus, lithium carbonate produces an antitumor effect by arresting cell cycle in the G2/M-phase and induction of apoptosis and autophagy in HCC-29 cells, which confirms the lithium potential as a promising drug for the treatment of hepatocellular carcinoma.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-020-05044-9