Nerve guidance conduit promoted peripheral nerve regeneration in rats

Nerve growth factor (NGF) is important for peripheral nerve regeneration. However, its short half‐life and rapid diffusion in body fluids limit its clinical efficacy. Collagen has favorable biocompatibility and biodegradability, and weak immunogenicity. Because it possesses an NGF binding domain, we...

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Bibliographic Details
Published in:Artificial organs 2021-06, Vol.45 (6), p.616-624
Main Authors: Long, Qingshan, Wu, Bingshan, Yang, Yu, Wang, Shanhong, Shen, Yiwen, Bao, Qinghua, Xu, Feng
Format: Article
Language:English
Subjects:
Autografts
Axon guidance
Binding
Biocompatibility
Biodegradability
Biodegradation
Biological activity
Body fluids
Cell proliferation
Collagen
Facial nerve
Growth factors
Heparin
Immunogenicity
Nerve growth factor
nerve guidance conduit
Nerve guides
nerve regeneration
peripheral nerve injury
Peripheral nerves
Recovery of function
Regeneration
Silicones
Surgery
Tubes
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Summary:Nerve growth factor (NGF) is important for peripheral nerve regeneration. However, its short half‐life and rapid diffusion in body fluids limit its clinical efficacy. Collagen has favorable biocompatibility and biodegradability, and weak immunogenicity. Because it possesses an NGF binding domain, we cross‐linked heparin to collagen tubes to construct nerve guidance conduits for delivering NGF. The conduits were implanted to bridge a facial nerve defect in rats. Histological and functional analyses were performed to assess the effect of the nerve guidance conduit on facial nerve regeneration. Heparin enhanced the binding of NGF to collagen while retaining its bioactivity. Also, the nerve guidance conduit significantly promoted axonal growth and Schwan cell proliferation at 12 weeks after surgery. The nerve regeneration and functional recovery outcomes using the nerve guidance conduit were similar to those of autologous nerve grafting. Therefore, the nerve guidance conduit may promote safer nerve regeneration. NGF was immobilized in collagen tube to promote nerve regeneration after facial nerve injury.
ISSN:0160-564X
1525-1594
DOI:10.1111/aor.13881