Single-Cell Infrared Microspectroscopy Quantifies Dynamic Heterogeneity of Mesenchymal Stem Cells during Adipogenic Differentiation

The central relevance of cellular heterogeneity to biological phenomena raises the rational needs for analytical techniques with single-cell resolution. Here, we developed a single-cell FTIR microspectroscopy-based method for the quantitative evaluation of cellular heterogeneity by calculating the c...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-01, Vol.93 (2), p.671-676
Main Authors: Wang, Yadi, Dai, Wentao, Liu, Zhixiao, Liu, Jixiang, Cheng, Jie, Li, Yuanyuan, Li, Xueling, Hu, Jun, Lü, Junhong
Format: Article
Language:English
Subjects:
Adipogenesis - physiology
Analytical chemistry
Cell differentiation
Cell Differentiation - physiology
Chemistry
Differentiation
Genotypes
Heterogeneity
Humans
Infrared spectra
Mesenchymal stem cells
Mesenchymal Stem Cells - physiology
Microspectrophotometry - methods
Phenotypes
Single-Cell Analysis - methods
Spectroscopy, Fourier Transform Infrared
Stem cells
Synchrotrons
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Summary:The central relevance of cellular heterogeneity to biological phenomena raises the rational needs for analytical techniques with single-cell resolution. Here, we developed a single-cell FTIR microspectroscopy-based method for the quantitative evaluation of cellular heterogeneity by calculating the cell-to-cell similarity distance of the infrared spectral data. Based on this method, we revealed the infrared phenotypes might reflect the dynamic heterogeneity changes in the cell population during the adipogenic differentiation of the human mesenchymal stem cells. These findings provide an alternative label-free optical approach for quantifying the cellular heterogeneity, and the combination with other single-cell analysis tools will be very helpful for understanding the genotype-to-phenotype relationship in cellular populations.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c04110