One-step acquirement of superior microbial communities from mesophilic digested sludge to upgrade anaerobic digestion

• Published in: Chemosphere (Oxford) 2021-01, Vol.263, p.128047-128047, Article 128047
• Main Authors: Wu, Li-Jie, Li, Xiao-Xiao, Yang, Fan, Zhou, Quan, Ren, Rui-Peng, Lyu, Yong-Kang
• Format: Article
• Language: English
• Subjects: Acclimation; Anaerobic digestion; Anaerobiosis; Archaea; Bioreactors; Hyperthermophilic; Methane; Microbial community; Microbiota; Organic solid waste; Sewage; Temperature; Waste activated sludge
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2020.128047

Anaerobic digestion is a promising waste-to-energy alternative technology. However, the efficiency upgrading for conventional mesophilic digestion of organic solid waste is always indispensable. Employing hyperthermophilic or thermophilic microbial community is one of the viable upgrading alternatives. Given the unavailability of the superior microbial communities, mesophilic digested sludge was used as inoculum, and instantly controlled at 70 °C and 55 °C for acclimation of hyperthermophilic and thermophilic inocula, respectively. Waste activated sludge was continuously and synchronously fed into two digesters. After one round, thermophilic digester achieved stable biogas production rate at 0.22 L L−1 d−1, with a methane proportion over 60%, whereas fluctuation was observed in the hyperthermophilic digester, and approximately triple time was needed to reach a relatively stable biogas production rate 0.12 L L−1 d−1. Nevertheless, higher hydrolysis ratio 24.4% was observed in the hyperthermophilic digester despite the lower biogas production. Therefore, methanogenesis step limited the whole anaerobic process for the hyperthermophilic digestion, and digestion at 70 °C was appropriate as a pre-fermentation stage to enhanced hydrolysis. The genus Methanothrix proportion in the thermophilic digester gradually decreased, while another acetoclastic genus Methanosarcina ultimately was acclimated to the dominant methanogen. In addition to Methanothrix, hydrogenotrophic archaea became competitive in the hyperthermophilic digester, with Methanothermobacter dominant at 22.6%. The genus Psychrobacter, affiliated to the phylum Proteobacteria could survive better than the others at 70 °C, with a final proportion of 62.5%. •Hyperthermophilic microbial community was established from mesophilic sludge.•The differences between digestion at 70 °C and 55 °C were profoundly discussed.•Hydrogenotrophic archaea Methanothermobacter was another dominant genus at 70 °C.•Higher hydrolysis ratio 24.4% was achieved in the hyperthermophilic digester.•Methanogenesis was the rate limiting step for hyperthermophilic digestion.