Hsa_circ_0001445 inhibits ox-LDL-induced HUVECs inflammation, oxidative stress and apoptosis by regulating miRNA-640

• Published in: Perfusion 2022-01, Vol.37 (1), p.86-94
• Main Authors: Cai, Yinlian, Xu, Ling, Xu, Chaoxiang, Wang, Yaoguo, Fan, Chenghui
• Format: Article
• Language: English
• Subjects: Apoptosis; Assaying; Atherosclerosis - genetics; Atherosclerosis - metabolism; Atherosclerosis - pathology; Cell growth; Cell proliferation; Cell viability; Damage; Flow cytometry; Glutathione; Glutathione peroxidase; Human Umbilical Vein Endothelial Cells - metabolism; Human Umbilical Vein Endothelial Cells - pathology; Humans; IL-1β; Inflammation; Inflammation - metabolism; Inflammatory response; Interleukin 16; Interleukin 6; Interleukins; Lipoproteins, LDL; Low density lipoprotein; Malondialdehyde; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Oxidation; Oxidative Stress; Peroxidase; Proteins; Ribonucleic acid; RNA; RNA, Circular - genetics; Superoxide dismutase; Tumor necrosis factor-TNF; Tumor necrosis factor-α
• ISSN: 0267-6591; 1477-111X
• DOI: 10.1177/0267659120979472

The role of Hsa_circ_0001445 in oxidation Low Lipoprotein (ox-LDL) induced HUVEC inflammatory damage remains poorly characterized. The present study investigated the performance of the circRNA Hsa_circ_0001445 on ox-LDL-induced HUVEC inflammatory damage. ox-LDL was employed to treat HUVECs and the expression of Hsa_circ_0001445 in cells were detected by qRT-PCR. Then, the overexpression plasmid of circ_0001445 was transfected into HUVECs. The Cell Counting Kit-8 assay was performed to detect cell viability, and the expression of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in treatment cells were measured using ELISAs. Furthermore, the oxidative stress kit was used to detect the levels of malondialdehyde, superoxide dismutase and glutathione peroxidase in treatment cells. Flow cytometry assay was applied to measure cell apoptosis, and the expressions of apoptosis-related protein were measured by western blot. The luciferase reporter assay was applied to confirm the target binding between Hsa_circ_0001445 and micro-RNA-640 (miRNA-640). Next, miRNA-640 mimic was transfected into ox-LDL-induced HUVECs, and then cell proliferation, expression level of inflammatory factors, oxidative stress and apoptosis level in treatment cells were assessed, with the expression of related proteins measured. The results revealed that the expression of Hsa_circ_0001445 was obviously downregulated in ox-LDL-induced HUVECs. Overexpression of Hsa_circ_0001445 promoted cell proliferation, inhibited ox-LDL-induced HUVEC inflammatory response, downregulate the expression of TNF-α, IL-1β and IL-16, overexpression of Hsa_circ_0001445 inhibited cell apoptosis. miRNA-640 was confirmed as a direct target of Hsa_circ_0001445, and miRNA-640 mimic reversed the effects of Hsa_circ_0001445 overexpression on ox-LDL-induced HUVECs. Our findings concluded that Hsa_circ_0001445 inhibits ox-LDL-induced HUVEC inflammation, oxidative stress and apoptosis by regulating miRNA-640.