Long noncoding RNA expression profiles in intermittent parathyroid hormone induced cementogenesis

• Published in: Genomics (San Diego, Calif.) Calif.), 2021-01, Vol.113 (1), p.217-228
• Main Authors: Li, Tiancheng, Wang, Han, Xia, Kai, Wu, Zuping, Liu, Ruojing, Yin, Xing, Zhou, Chenchen, Li, Yuyu, Zou, Shujuan
• Format: Article
• Language: English
• Subjects: Adenomatous Polyposis Coli Protein; Animals; Axin Protein - genetics; Axin Protein - metabolism; beta Catenin - genetics; beta Catenin - metabolism; Cell Line; Cementoblast; Cementogenesis; Dental Cementum - metabolism; Long noncoding RNA; Mice; Microarray; Osteoblasts - metabolism; Parathyroid hormone; Parathyroid Hormone - metabolism; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Transcriptome; Wnt signaling; Wnt Signaling Pathway
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2020.12.012

The aim of this study was to explore the involvement of long noncoding RNAs (lncRNAs) during intermittent parathyroid hormone (PTH) induced cementogenesis. Expression profiles of lncRNAs and mRNAs were obtained using high-throughput microarray. Gene Ontology enrichment analysis, Kyoto Encyclopedia of Genes and Genomes pathway analysis, and coding-noncoding gene coexpression networks construction were performed. We identified 190 lncRNAs and 135 mRNAs that were differentially expressed during intermittent PTH-induced cementogenesis. In this process, the Wnt signaling pathway was negatively regulated, and eight lncRNAs were identified as possible core regulators of Wnt signaling. Based on the results of microarrray analysis, we further verified the repressed expression of Wnt signaling crucial components β-catenin, APC and Axin2. Above all, we speculated that lncRNAs may play important roles in PTH-induced cementogenesis via the negative regulation of Wnt pathway. •Differentially expressed lncRNAs were identified during PTH-induced cementogenesis.•Wnt crucial components β-catenin, APC and Axin2 were repressed in this process.•Eight lncRNAs were identified as possible core regulators of Wnt signaling.•lncRNAs played crucial roles in cementogenesis via negative regulation of Wnt pathway.