DNA repair, NFKβ, and TP53 polymorphisms associated with potentially malignant disorders and oral squamous cell carcinoma in Argentine patients

• Published in: Oral surgery, oral medicine, oral pathology and oral radiology oral medicine, oral pathology and oral radiology, 2021-03, Vol.131 (3), p.339-346
• Main Authors: Galíndez, María Fernanda, Carrica, Andrés, Zarate, Ana María, Secchi, Dante, Don, Julieta, Barra, José Luis, Brunotto, Mabel
• Format: Article
• Language: English
• Subjects: Carcinoma, Squamous Cell - genetics; Case-Control Studies; Cross-Sectional Studies; DNA Repair; Genetic Predisposition to Disease; Head and Neck Neoplasms; Humans; Mouth Neoplasms - genetics; Polymorphism, Single Nucleotide - genetics; Squamous Cell Carcinoma of Head and Neck; Tumor Suppressor Protein p53
• ISSN: 2212-4403; 2212-4411
• DOI: 10.1016/j.oooo.2020.11.004

An important strategy in cancer prevention is to identify individual susceptibilities for cancer development through the genomic profile. Developing countries such as Argentina have no data on genetic composition. The aim of this study was to evaluate the single nucleotide polymorphisms of genes related to DNA repair (XCCR3, XPD), cell cycle arrest/apoptosis (TP53), and inflammation (NFKβ) of patients with precancer and oral cancer and to contribute to recognizing potential risk of developing these pathologies, and incorporate the risk patients into a clinical follow-up program in Córdoba, Argentina. A cross-sectional study was performed on 140 patients with oral squamous cell carcinoma (OSCC), oral potentially malignant disorders (OPMDs), and controls. Genotyping of single nucleotide polymorphisms was performed using allele-specific polymerase chain reaction or restriction fragment length polymorphism techniques. The variables were evaluated by bivariate and multivariate statistical methods, with P < .05 statistically significant. The multiple correspondence analyses showed that patients with OSCC are clustered with the T allele of XRCC3 T241 M and the C allele of TP53 R72 P, and patients with OPMDs are clustered with the T allele of NFKβ-519. Our preliminary results showed that the C allele of the Pro72 variant of TP53 was related to OSSC and OPMD, and the T allele of NFKβ-519 is related to OPMDs in Argentine patients.