Induction of scaffold angiogenesis by recipient vasculature precision micropuncture

The success of engineered tissues continues to be limited by time to vascularization and perfusion. Here, we studied the effects of precision injury to a recipient macrovasculature in promoting neovessel formation in an adjacently placed scaffold. Segmental 60 μm diameter micropunctures (MP) were cr...

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Bibliographic Details
Published in:Microvascular research 2021-03, Vol.134, p.104121-104121, Article 104121
Main Authors: Hancock, Patrick C., Koduru, Srinivas V., Sun, Mingjie, Ravnic, Dino J.
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Collagen
Collagen - metabolism
Femoral Artery - metabolism
Femoral Vein - metabolism
Graft
Hindlimb - blood supply
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Micropuncture
Neovascularization, Physiologic
Punctures
Rats
Rats, Sprague-Dawley
Receptor, TIE-2 - metabolism
Scaffold
Signal Transduction
Surgery
Time Factors
Tissue Engineering
Tissue Scaffolds
Vascular Endothelial Growth Factor Receptor-2 - metabolism
Vascularization
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Summary:The success of engineered tissues continues to be limited by time to vascularization and perfusion. Here, we studied the effects of precision injury to a recipient macrovasculature in promoting neovessel formation in an adjacently placed scaffold. Segmental 60 μm diameter micropunctures (MP) were created in the recipient rat femoral artery and vein followed by coverage with a simple collagen scaffold. Scaffolds were harvested at 24, 48, 72, and 96 h post-implantation for detailed analysis. Those placed on top of an MP segment showed an earlier and more robust cellular infiltration, including both endothelial cells (CD31) and macrophages (F4/80), compared to internal non-micropunctured control limbs (p 
ISSN:0026-2862
1095-9319
DOI:10.1016/j.mvr.2020.104121