Rapid determination of isoflavones and other bioactive compounds in soybean using SWATH-MS

• Published in: Analytica chimica acta 2020-03, Vol.1103, p.122-133
• Main Authors: Chien, Han-Ju, Wang, Chang-Sheng, Chen, Yu-Hsun, Toh, Jie-Teng, Zheng, Yi-Feng, Hong, Xiang-Gui, Lin, Hung-Yu, Lai, Chien-Chen
• Format: Article
• Language: English
• Subjects: Amino acid; Amino Acids - analysis; Chromatography, High Pressure Liquid; Glycine max - chemistry; High-throughput; Isoflavone; Isoflavones - analysis; Reproducibility of Results; Saponin; Saponins - analysis; Soybean; SWATH-MS; Tandem Mass Spectrometry - methods
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2019.12.054

Isoflavones are the major bioactive components in soybeans. Sequential window acquisition of all theoretical fragment ions (SWATH) is a kind of data-independent acquisition (DIA), such that all fragments of each precursor will be preserved in a SWATH-Mass Spectrometry (SWATH-MS) run. In this study, a high-throughput SWATH-MS method for the determination of 12 isoflavones in soybeans was established. Furthermore, amino acids, saponins can be semi-quantitated from the same SWATH-MS data. Combination of targeted quantification and untargeted profiling with SWATH, all bioactive compounds were analyzed within 5 min in 10 min run time, and the method had good linear regression with r2 > 0.99. The precisions (RSD %) of the intra-day and inter-day analyses ranged from 2.11% to 18.7%, and the accuracies (RE%) ranged from −14.39% to 17.48%. The matrix effect ranged from 88.66% to 114.82%. Moreover, 7 varieties of soybeans were analyzed and compared with this robust screening method. A rapid SWATH-MS method for the quantitation of 12 isoflavones and the semi-quantitation of amino acids, saponins in soybeans. [Display omitted] •High throughput SWATH-MS method for the determination of 12 isoflavones in soybeans.•Amino acids, saponin Ba, Bb, and Bc can be semi-quantified from the SWATH-MS data.•All bioactive compounds were quantified within 5 min in 10 min run time.•This approach provides good quantitative linearity and reproducibility.•7 varieties of soybeans were compared with this robust screening method.