LncRNA SNHG5 upregulation induced by YY1 contributes to angiogenesis via miR-26b/CTGF/VEGFA axis in acute myelogenous leukemia

• Published in: Laboratory investigation 2021-03, Vol.101 (3), p.341-352
• Main Authors: Li, Zhen-Jiang, Cheng, Jing, Song, Yuan, Li, Hui-Hui, Zheng, Ji-Fu
• Format: Article
• Language: English
• Subjects: 631/67/1990/283/1897; 692/699/67/1990/283/1897; Acute myeloid leukemia; Adult; Angiogenesis; Cell cycle; Cell Line, Tumor; Cell migration; Cell Survival; Connective tissue; Connective tissue growth factor; Connective Tissue Growth Factor - genetics; Connective Tissue Growth Factor - metabolism; Connective tissues; Endothelial cells; Female; Growth factors; Humans; Laboratory Medicine; Leukemia; Leukemia, Myeloid, Acute - genetics; Leukemia, Myeloid, Acute - metabolism; Male; Medicine; Medicine & Public Health; MicroRNAs - genetics; MicroRNAs - metabolism; Molecular modelling; Myeloid leukemia; Neovascularization, Pathologic - genetics; Nucleoli; Pathogenesis; Pathology; Ribonucleic acid; RNA; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Signal Transduction - genetics; snoRNA; Tumor cell lines; Umbilical vein; Up-regulation; Up-Regulation - genetics; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism; YY1 protein; YY1 Transcription Factor - genetics; YY1 Transcription Factor - metabolism
• ISSN: 0023-6837; 1530-0307
• DOI: 10.1038/s41374-020-00519-9

Acute myelogenous leukemia (AML) is the most common acute leukemia in adults. Despite great progress has been made in this field, the pathogenesis of AML is still not fully understood. We report here the biological role of lncRNA small nucleolar RNA host gene 5 (SNHG5) in the pathogenesis of AML and the underlying mechanisms. The results showed that lncRNA SNHG5 was highly expressed in AML cancer cell lines. In vitro studies displayed that inhibition of SNHG5 with shRNA resulted in suppression of survival, cell cycle progression, migration/invasion of AML and capacity of adhesion and angiogenesis in human umbilical vein endothelial cells. Mechanistic studies revealed a SNHG5/miR-26b/connective tissue growth factor (CTGF)/vascular endothelial growth factor A (VEGFA) axis in the regulation of AML angiogenesis. Finally, Yin Yang 1 (YY1) was found to transactivate and interact with SNHG5 promoter, leading to the upregulation of SNHG5 in AML. Collectively, upregulation of lncRNA SNHG5 mediated by YY1, activates CTGF/VEGFA via targeting miR-26b to regulate angiogenesis of AML. Our work provides new insights into the molecular mechanisms of AML. This study reveals that upregulation of lncRNA small nucleolar RNA host gene 5 (SNHG5), mediated by Yin Yang 1 (YY1), activates connective tissue growth factor (CTGF)/vascular endothelial growth factor (VEGFA) axis via targeting miR-26b to regulate angiogenesis of acute myelogenous leukemia (AML), providing new insights into mechanisms of AML.