Comparison of the efficacy of metagenomic next-generation sequencing and Xpert MTB/RIF in the diagnosis of tuberculous meningitis

Metagenomic next-generation sequencing (mNGS) is an emerging sequence-based method that detects genomic information of pathogenic microorganisms from a wide range of clinical specimens. The mNGS has moderate sensitivity and very high specificity for tuberculous meningitis, and the validity of mNGS w...

Full description

Saved in:
Bibliographic Details
Published in:Journal of microbiological methods 2021-01, Vol.180, p.106124-106124, Article 106124
Main Authors: Yu, Guocan, Wang, Xiaodan, Zhu, Pengfei, Shen, Yanqin, Zhao, Wuchen, Zhou, Lihong
Format: Article
Language:English
Subjects:
mNGS
Mycobacterium tuberculosis
Tuberculous meningitis
Xpert MTB/RIF
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Metagenomic next-generation sequencing (mNGS) is an emerging sequence-based method that detects genomic information of pathogenic microorganisms from a wide range of clinical specimens. The mNGS has moderate sensitivity and very high specificity for tuberculous meningitis, and the validity of mNGS was higher than that of Xpert MTB/RIF. •mNGS showed moderate sensitivity and very high specificity for TBM.•mNGS was more effective than Xpert, but the difference was insignificant.•mNGS can detect a variety of pathogenic microorganisms.•mNGS can be used as an initial test for unexplained meningitis.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2020.106124