Mechanism evaluation for an amino acid substitution p.Y246C of B‐glycosyltransferase enzyme with Bweak phenotype

• Published in: Vox sanguinis 2021-04, Vol.116 (4), p.464-470
• Main Authors: Ying, Yanling, Hong, Xiaozhen, Xu, Xianguo, Zhang, Jingjing, He, Ji, Zhu, Faming, Xie, Xinyou
• Format: Article
• Language: English
• Subjects: ABO subgroup; ABO system; Alleles; Amino acid substitution; Amino acids; Antigens; Biotechnology; Cell lines; Enzymatic activity; Enzyme activity; Enzymes; Free energy; Genotypes; Glycosyltransferase; Haplotypes; Hydrogen bonds; Impact prediction; in vitro expression; Phenotypes; Polymerase chain reaction; protein stability; Proteins; Serological tests; Subgroups; Substitution reactions
• ISSN: 0042-9007; 1423-0410
• DOI: 10.1111/vox.13041

Background The amino acid substitutions caused by ABO gene variants are usually predicted to impact the glycosyltransferase function. Here, the effect of an amino acid substitution in the vicinity of the catalytic active region of the B‐glycosyltransferase was explored in vitro and in silico study, which is important for further recognizing the ABO subgroup. Methods The ABO serological tests were performed by the routine methods. The ABO genotype was analyzed by polymerase chain reaction and sequenced bidirectionally. The haplotype of the variant allele was separated using single‐strand amplification and sequencing with allele‐specific primers. Stably expression cell lines with variant were constructed for study in vitro. 3D structure of the B‐glycosyltransferase (GTB) variant was simulated by PyMOL software. The free energy change (ΔΔG) was calculated by FoldX. Results A variant c.737A > G was identified in a Chinese individual with Bweak phenotype, which led to an amino acid substitution p.Y246C in the vicinity of the catalytic active region of GTB enzyme. The stably expression cell lines with variant and wild type were successfully established and showed that the variant caused a decrease in protein levels and/or enzyme activity. The 3D structural of the GTB modelling found the amino acid substitution p.Y246C caused the hydrogen bond of the protein changes. Meanwhile, the free energy change (ΔΔG) value predicted the destabilizing effect on the variant GTB. Discussion The p.Y246C variant in the vicinity of the enzyme active centre reduced the antigen expression because of greatly destabilizing effect on the GTB variant.