Pulsed Interleaved MINFLUX

We introduce p-MINFLUX, a new implementation of the highly photon-efficient single-molecule localization method with a simplified experimental setup and additional fluorescence lifetime information. In contrast to the original MINFLUX implementation, p-MINFLUX uses interleaved laser pulses to delive...

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Published in:Nano letters 2021-01, Vol.21 (1), p.840-846
Main Authors: Masullo, Luciano A, Steiner, Florian, Zähringer, Jonas, Lopez, Lucía F, Bohlen, Johann, Richter, Lars, Cole, Fiona, Tinnefeld, Philip, Stefani, Fernando D
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cited_by cdi_FETCH-LOGICAL-c353t-836d7dbd809b349029c1f58e5228706e7f81c1cb667e478b895d0f81e37d8cce3
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container_end_page 846
container_issue 1
container_start_page 840
container_title Nano letters
container_volume 21
creator Masullo, Luciano A
Steiner, Florian
Zähringer, Jonas
Lopez, Lucía F
Bohlen, Johann
Richter, Lars
Cole, Fiona
Tinnefeld, Philip
Stefani, Fernando D
description We introduce p-MINFLUX, a new implementation of the highly photon-efficient single-molecule localization method with a simplified experimental setup and additional fluorescence lifetime information. In contrast to the original MINFLUX implementation, p-MINFLUX uses interleaved laser pulses to deliver the doughnut-shaped excitation foci at a maximum repetition rate. Using both static and dynamic DNA origami model systems, we demonstrate the performance of p-MINFLUX for single-molecule localization nanoscopy and tracking, respectively. p-MINFLUX delivers 1-2 nm localization precision with 2000-1000 photon counts. In addition, p-MINFLUX gives access to the fluorescence lifetime enabling multiplexing and super-resolved lifetime imaging. p-MINFLUX should help to unlock the full potential of innovative single-molecule localization schemes.
doi_str_mv 10.1021/acs.nanolett.0c04600
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title Pulsed Interleaved MINFLUX
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