Miniaturized skeletal muscle tissue fabrication for measuring contractile activity

The contractile function of skeletal muscle is essential for maintaining the vital activity of life. Muscular diseases such as muscular dystrophy severely compromise the quality of life of patients and ultimately lead to death. There is therefore an urgent need to develop therapeutic agents for thes...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2021-04, Vol.131 (4), p.434-441
Main Authors: Yoshioka, Kantaro, Ito, Akira, Arifuzzaman, Md, Yoshigai, Taichi, Fan, Fangming, Sato, Kei-ichiro, Shimizu, Kazunori, Kawabe, Yoshinori, Kamihira, Masamichi
Format: Article
Language:English
Subjects:
Animals
C2C12 cell
Cell Differentiation
Cell Line
Drug screening
Human iPS cell
Induced Pluripotent Stem Cells - cytology
l-Carnosine
Mice
Muscle Contraction
Muscle, Skeletal - cytology
Myoblasts - cytology
Polydimethylsiloxane device
Skeletal muscle tissue
Tissue engineering
Tissue Engineering - methods
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Summary:The contractile function of skeletal muscle is essential for maintaining the vital activity of life. Muscular diseases such as muscular dystrophy severely compromise the quality of life of patients and ultimately lead to death. There is therefore an urgent need to develop therapeutic agents for these diseases. In a previous study, we showed that three-dimensional skeletal muscle tissues fabricated using the magnetic force-based tissue engineering technique exhibited contractile activity, and that drug effects could be evaluated based on the contractile activity of the skeletal muscle tissues. However, the reported method requires a large number of cells and the tissue preparation procedure is complex. It is therefore necessary to improve the tissue preparation method. In this study, a miniature device made of polydimethylsiloxane was used to simplify the production of contracting skeletal muscle tissues applicable to high-throughput screening. The effects of model drugs on the contractile force generation of skeletal muscle tissues prepared from mouse C2C12 myoblast and human induced pluripotent stem cells were evaluated using the miniature muscle device. The results indicated that the muscle device system could provide a useful tool for drug screening.
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2020.11.014