Lignocellulosic fibres surface interactions in enzymatic reaction using data-mining

• Published in: Carbohydrate polymers 2021-02, Vol.254, p.117412-117412, Article 117412
• Main Authors: Morin, Sophie, Lecart, Brieuc, Lang, Mylène, Richel, Aurore
• Format: Article
• Language: English
• Subjects: Cannabis - chemistry; Coumaric Acids - chemistry; Data Mining - methods; Dimerization; ferulic acid; Flax - chemistry; Hydrogen-Ion Concentration; Laccase; Laccase - chemistry; Lignin - chemistry; lignocellulosic fibres; Mannans - chemistry; Plant Extracts - chemistry; Surface Properties; Temperature; Xylans
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2020.117412

•Presence of lignocellulosic materials affects the structure and abundancy of laccase and trans-ferulic acid reactions products•Ferulic acid dimers and trimers ongoing carboxylation, hydroxylation, methylation, and methoxylation addition and depletion were detected•Structure of the ferulic acid derivatives is temperature-dependent•Ferulic acid derivatives in presence of lignocellulosic fibres are correlated to the reaction duration•Constituents of the surface of the fibres were linked to the formation of the ferulic acid derivatives Lignocellulosic fibres modification focused so far essentially on the resulting material properties to create functional fibres instead of determining the reaction influencing parameters. Using a data-mining algorithm, surface chemical composition of the fibres after modifications was compared to multiple signals. A 24 h reaction at either 25 °C or 60 °C, pH5 was conducted in presence of trans-ferulic acid, laccase, and lignocellulosic fibres (flax, hemp, or cellulose) having different chemical surface composition. Dimers and trimers were detected in variable concentrations in the reaction filtrate and extractive. At 25 °C, crystalline cellulose, amorphous cellulose, xylans, mannans, and lignins were well correlated to specific reaction products while at 60 °C, only lignins and xylan were found correlated to reaction products. Fibres surface composition affected the extractive profile. Lignocellulosic surface composition influence on the product formed was unveiled using a data mining approach. This study presents a way to unveil non-evident chemical interface interaction in reactions.