Polarization, migration, and homotypical interactions among prostatic smooth muscle cells in a laminin 111‐rich extracellular matrix

Prostate cancer is a life‐threatening condition worldwide. As the tumor progresses, smooth muscle cells (SMCs) become atrophic/dedifferentiated, within a series of stromal changes named stromal reaction. Here, we tested whether a laminin 111‐rich extracellular matrix (Lr‐ECM) could affect SMCs pheno...

Full description

Saved in:
Bibliographic Details
Published in:Cell biology international 2021-04, Vol.45 (4), p.882-889
Main Authors: Osório, Daniel A., Consonni, Silvio R., Santos, Aline M., Carvalho, Hernandes F.
Format: Article
Language:English
Subjects:
Autophagy
cell adhesions
Cell junctions
cell polarization
Epithelium
Extracellular matrix
FAK
Focal adhesion kinase
Immunoblotting
Immunohistochemistry
Laminin
laminin 111‐rich ECM
Microscopy
Phagocytosis
Phenotypes
Polarization
Polymerase chain reaction
Prostate cancer
Reverse transcription
Smooth muscle
smooth muscle cells
Transmission electron microscopy
α‐smooth muscle actin
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Prostate cancer is a life‐threatening condition worldwide. As the tumor progresses, smooth muscle cells (SMCs) become atrophic/dedifferentiated, within a series of stromal changes named stromal reaction. Here, we tested whether a laminin 111‐rich extracellular matrix (Lr‐ECM) could affect SMCs phenotype and differentiation status. Using time‐lapse microscopy, image analyses, quantitative real‐time reverse transcription polymerase chain reaction, immunohistochemistry and immunoblotting, and transmission electron microscopy, we showed that SMCs acquires a migratory behavior with a decreased expression of differentiation markers and relocation of focal adhesion kinase. SMCs set homotypic cell junctions and were active in autophagy/phagocytosis. Analysis of the migratory behavior showed that SMCs polarized and migrated toward each other, recognizing long‐distance signals such as matrix tensioning. However, half of the cell population were immotile, irrespective of the nearest neighbor distance, suggesting they do not engage in productive interactions, possibly as a result of back‐to‐back positioning. In conclusion, the Lr‐ECM, mimics the effects of the proliferating and infiltrating tumor epithelium, causing SMCs phenotypical change similar to that observed in the stromal reaction, in addition to a hitherto undescribed, stereotyped pattern of cell motility resulting from cell polarization.
ISSN:1065-6995
1095-8355
DOI:10.1002/cbin.11535