A highly sensitive multiplex immunoassay for inflammatory cytokines in dried blood spots

Objectives Inflammatory cytokines are key regulators of inflammation, but current measurement approaches require venous blood to quantify low circulating concentrations associated with chronic, low‐grade inflammation. This article describes a highly sensitive multiplex immunoassay protocol for the m...

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Bibliographic Details
Published in:American journal of human biology 2021-11, Vol.33 (6), p.e23558-n/a
Main Authors: McDade, Thomas W., Miller, Aaron, Tran, Tina T., Borders, Ann E. B., Miller, Greg
Format: Article
Language:English
Subjects:
Blood circulation
Coefficient of variation
Cytokines
Cytokines - analysis
Dilution
Dried Blood Spot Testing
Electrochemiluminescence
Erythrocytes
Humans
Immune response
Immunoassay
Inflammation
Interleukin 1
Interleukin 10
Interleukin 6
Interleukin 8
Linearity
Low concentrations
Multiplexing
Reliability analysis
Reproducibility of Results
Tumor necrosis factor-α
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Summary:Objectives Inflammatory cytokines are key regulators of inflammation, but current measurement approaches require venous blood to quantify low circulating concentrations associated with chronic, low‐grade inflammation. This article describes a highly sensitive multiplex immunoassay protocol for the measurement of IL6, IL8, IL10, and TNFα in finger stick dried blood spot (DBS) samples. Methods The protocol uses a multiplex electrochemiluminescent immunoassay platform. The following measures of assay performance were evaluated: reliability (inter‐assay percent coefficient of variation; %CV), precision (intra‐assay %CV), lower limit of detection (LLD), linearity of dilution, and agreement with results from matched plasma samples. Results Analysis of three control samples across the assay range indicated an acceptable level of precision and reliability for each cytokine. Linearity of dilution returned average values that ranged from 104.1 to 127.6% of expected. Lower limits of detection for IL6, IL8, and IL10 were
ISSN:1042-0533
1520-6300
DOI:10.1002/ajhb.23558