Molecularly imprinted photo-electrochemical sensor for hemoglobin detection based on titanium dioxide nanotube arrays loaded with CdS quantum dots

• Published in: Talanta (Oxford) 2021-03, Vol.224, p.121924-121924, Article 121924
• Main Authors: Gao, Bowen, Liang, Zhishan, Han, Dongxue, Han, Fangjie, Fu, Wencai, Wang, Wei, Liu, Zhenbang, Niu, Li
• Format: Article
• Language: English
• Subjects: CdS/TiO2 nanocomposites; Electrochemical Techniques; Hemoglobin; Hemoglobins; Humans; Hydrogen Peroxide; Limit of Detection; Molecular Imprinting; Molecularly imprinted polymer; Nanotubes; Photo-electrochemical sensor; Quantum Dots; Titanium
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2020.121924

A novel molecularly imprinted photo-electrochemical sensor based on CdS/TiO2 nanocomposites was constructed for precisely detection of hemoglobin under visible light irradiation. CdS quantum dots were decorated on the surface of TiO2 nanorod arrays to form a heterojunction, which could enhance the charge-transfer efficiency for visible light and further increase the photo-generated current of the sensor. The molecularly imprinted polymer film assembled by dopamine monomer had achieved excellent performance for specifically binding with human hemoglobin. The hemoglobin bound on the sensor could catalyze the oxidation reaction of 4-chloro-1-naphthol by H2O2, generating insoluble product on the sensor surface and triggering an obviously decrease on photocurrent. The molecularly imprinted photo-electrochemical sensor exhibited excellent sensitivity, selectivity and stability for the detection of human hemoglobin. The sensor had a linear range from 0.01 to 100 ng mL−1 with a detection limit of 0.53 pg/mL (S/N = 3). Furthermore, the sensor was successfully applied on the analysis of human hemoglobin in the urine samples. [Display omitted] •The modification of MIP film on the PEC sensor is an effective strategy for the sensitive detection of macromolecules by the PEC method.•It is a simple and low-cost technique for the construction of MIP-PEC sensor for hemoglobin detection based on dopamine polymerization.•The PEC sensor exhibited excellent sensitivity, selectivity and stability, the detection range was 0.01–100 ng mL−1 with an ultralow detection limit of 0.53.•A MIP-PEC sensor for Hb detection with excellent performance was constructed and employed for the detection in urine sample.