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Ultrasensitive electrochemical assay for microRNA-21 based on CRISPR/Cas13a-assisted catalytic hairpin assembly

MicroRNAs (miRNAs) are related to many biological processes and regarded as biomarkers of disease. Rapid, sensitive, and specific methods for miRNA assay are very important for early disease diagnostic and therapy. In the present work, an ultrasensitive electrochemical biosensing platform has been d...

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Bibliographic Details
Published in:Talanta (Oxford) 2021-03, Vol.224, p.121878-121878, Article 121878
Main Authors: Cui, Ying, Fan, Shanji, Yuan, Ze, Song, Minghui, Hu, Jiawen, Qian, Dong, Zhen, Deshuai, Li, Junhua, Zhu, Baode
Format: Article
Language:English
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Summary:MicroRNAs (miRNAs) are related to many biological processes and regarded as biomarkers of disease. Rapid, sensitive, and specific methods for miRNA assay are very important for early disease diagnostic and therapy. In the present work, an ultrasensitive electrochemical biosensing platform has been developed for miRNA-21 assay by combining CRISPR-Cas13a system and catalytic hairpin assembly (CHA). In the presence of miRNA-21, it would hybridize with the spacer region of Cas13a/crRNA duplex to activate the cleavage activity of CRISPR-Cas13a system, leading to the release of initiator of CHA to generate amplified electrochemical signals. Base on the CRISPR-Cas13a-mediated cascade signal amplification strategy, the developed electrochemical biosensing platform exhibited high sensitivity with a low detection limit of 2.6 fM (S/N = 3), indicating that the platform has great potential for application in early clinical diagnostic. [Display omitted] •An CRISPR/Cas13a-assisted catalytic hairpin assembly has been developed.•The platform can detect miRNA-21 with a cascade amplified signal.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2020.121878