A smartphone-assisted portable biosensor using laccase-mineral hybrid microflowers for colorimetric determination of epinephrine

• Published in: Talanta (Oxford) 2021-03, Vol.224, p.121840-121840, Article 121840
• Main Authors: Zhang, Miaorong, Zhang, Yan, Yang, Chuankai, Ma, Chunyun, Tang, Jianguo
• Format: Article
• Language: English
• Subjects: Biomineralization; Biosensing Techniques; Colorimetric detection; Colorimetry; Epinephrine; Laccase; Minerals; Smartphone
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2020.121840

A novel smartphone-assisted portable biosensor based on laccase-mineral hybrid microflowers (La-HMFs) was applied for real-time, accurate and reliable quantification of epinephrine (EP). La-HMFs with flower-like hierarchical nanostructure was synthesized via biomineralization using Cu3(PO4)2⋅3H2O as the mineral. Characterization results revealed laccase molecules as the framework were immobilized within La-HMFs. Smartphone-assisted colorimetric assays showed wide linear detection range (1–400 μM) and favorable anti-interference ability for EP detection, and the detection limit was 0.6 μM. Further, due to the protective effect of Cu3(PO4)2⋅3H2O, the immobilized laccase exhibited good stability and desirable reusability. A novel smartphone-assisted portable biosensor based on laccase-mineral hybrid microflowers was applied for real-time, accurate and reliable quantification of epinephrine. [Display omitted] •A smartphone-assisted portable biosensor was constructed for epinephrine detection.•Laccase-mineral hybrid microflowers were synthesized via biomineralization.•This easy-to-operate biosensor had favorable sensitivity for epinephrine detection.•Laccase-mineral hybrid microflowers have desirable stability and reusability.