Mitochondrion-targeting near-infrared fluorescent probe for detecting intracellular nanomolar level hydrogen sulfide with high recognition rate

Hydrogen sulfide (H 2 S) typically plays biphasic biological roles in living organisms with subnormal H 2 S exerting cytoprotective effects such as participating in cardioprotective signaling pathways while H 2 S with higher-than-normal concentrations in localized tissues acting the opposite way suc...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2021-02, Vol.413 (4), p.1215-1224
Main Authors: Zhang, Kaiquan, Meng, Jiaqi, Bao, Weier, Liu, Ming, Wang, Xuefei, Tian, Zhiyuan
Format: Article
Language:English
Subjects:
Analysis
Analytical Chemistry
Biochemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Fluorescence
Fluorescent indicators
Food Science
Hydrogen sulfide
I.R. radiation
Identification and classification
Intracellular
Laboratory Medicine
Microenvironments
Mitochondria
Molecules
Monitoring/Environmental Analysis
Physiology
Recognition
Research Paper
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Summary:Hydrogen sulfide (H 2 S) typically plays biphasic biological roles in living organisms with subnormal H 2 S exerting cytoprotective effects such as participating in cardioprotective signaling pathways while H 2 S with higher-than-normal concentrations in localized tissues acting the opposite way such as inhibiting mitochondrial respiration. Such concentration-dependent biological and pathological roles of H 2 S with the wide involvement of mitochondria and the elusive feature of H 2 S definitely highlight the vital significance of fast and precise estimation of the physiological level of H 2 S in specific microenvironments, particularly within cellular mitochondria. In this work, we developed a new type of fluorescent probe (QcyCHO) featured with H 2 S-triggered off-to-on near-infrared (NIR) fluorescence conversion within ~ 10 min, limit of detection (LOD) down to 8.3 nM, and high recognition specificity over other similarly interfering species. The ideal mitochondrion-targeting ability, high recognition specificity over typical interfering substances and other physiologically relevant species, and the ability for mapping intracellular H 2 S in living cells of QcyCHO probe were also unequivocally confirmed, which imply its potential for shedding light on the biology of H 2 S and therapeutic development in H 2 S-associated diseases by identifying the specific physiological stimuli inducing H 2 S production and determining the levels of H 2 S at the location and time of stimulation. Graphical abstract
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-020-03086-6