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Ureaplasma diversum and Ureaplasma sp. in nasal cavity of pigs: Distribution among herds and individual frequency based on pooled samples

The role of Ureaplasma diversum in the porcine respiratory disease complex (PRDC), its circulation among herds and prevalence in live pigs is unknown; thus, the objectives of this study were: to determine the presence of U. diversum in indoor intensive pig herds and to determine the individual frequ...

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Bibliographic Details
Published in:Revista argentina de microbiología 2021-04, Vol.53 (2), p.141-144
Main Authors: Seitz, Jimena, Vicharelli, Gastón, García, Virginia, Vissio, Claudina, Estanguet, Abel, Parada, Julián, Carranza, Alicia, Di Cola, Gabriel, Tamiozzo, Pablo
Format: Article
Language:English
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Summary:The role of Ureaplasma diversum in the porcine respiratory disease complex (PRDC), its circulation among herds and prevalence in live pigs is unknown; thus, the objectives of this study were: to determine the presence of U. diversum in indoor intensive pig herds and to determine the individual frequency of pigs with U. diversum from pooled samples. A cross sectional study was carried out in 16 indoor intensive herds from Córdoba and La Pampa provinces, collecting eight nasal swabs specimens that were further processed by two pools of four specimens each by a PCR targeting a fragment of 16S ribosomal RNA gene. Four PCR products were sequenced and aligned against data bases. U. diversum – Ureaplasma sp. were detected in 56.3% of the analyzed herds, with 16.5% (95% CI 8.2–30.8) of positive pigs. It was concluded that U. diversum – Ureaplasma sp. are present in the nasal cavity of live pigs, being widely distributed among herds. La participación de Ureaplasma diversum (U. diversum) en el complejo de las enfermedades respiratorias porcinas (CERP), su circulación entre las piaras y la prevalencia en cerdos vivos se desconoce. Este estudio tuvo por objetivos determinar la presencia de U. diversum en criaderos intensivos de cerdos y la frecuencia individual de cerdos portadores de dicho agente, a partir de muestras agrupadas. Se realizó un estudio transversal en 16 granjas confinadas de las provincias de Córdoba y La Pampa, en el que se recogieron ocho muestras de hisopos nasales que se procesaron posteriormente en dos grupos de cuatro muestras cada uno, mediante una PCR dirigida a un fragmento del gen del 16S ARN ribosomal. Se secuenciaron cuatro productos de la PCR y se les alineó contra las bases de datos. Se detectó U. diversum-Ureaplasma sp. en el 56,3% de las granjas, con 16,5% (IC del 95%: 8,2-30,8) de cerdos positivos. Se concluyó que U. diversum-Ureaplasma sp. están presentes en la cavidad nasal de cerdos vivos, con amplia distribución en las granjas.
ISSN:0325-7541
DOI:10.1016/j.ram.2020.11.002