Protein mass spectrometry reveals lycorine exerting anti-multiple-myeloma effect by acting on VDAC2 and causing mitochondrial abnormalities

Objective Two-dimensional electrophoresis (2-DE) and MALDI-TOF/TOF mass spectrometry were performed to compare the proteomic alterations of lycorine-treated and control cells to further investigate the anti-multiple myeloma (MM) mechanisms of lycorine. Results Mass spectrometry results showed that a...

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Bibliographic Details
Published in:Biotechnology letters 2021-03, Vol.43 (3), p.537-546
Main Authors: Yi, Hui, Shen, Xiaokai, Wang, Haiqin, Luo, Saiqun, Guo, Wancheng, Chen, Peng, Hu, Lei, Liang, Long, Gong, Yanfei, Xiao, Xiaojuan, Liu, Jing
Format: Article
Language:English
Subjects:
Abnormalities
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biotechnology
Electrophoresis
Ions
Life Sciences
Localization
Mass spectrometry
Mass spectroscopy
Membrane potential
Membranes
Microbiology
Mitochondria
Multiple myeloma
Original Research Paper
Proteins
Scientific imaging
Spectroscopy
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Summary:Objective Two-dimensional electrophoresis (2-DE) and MALDI-TOF/TOF mass spectrometry were performed to compare the proteomic alterations of lycorine-treated and control cells to further investigate the anti-multiple myeloma (MM) mechanisms of lycorine. Results Mass spectrometry results showed that after lycorine treatment of MM cells, 42% of the differentially expressed proteins had subcellular localization, mainly, on mitochondria. Voltage-dependent anion-selective channel protein 2 (VDAC2), the most abundant protein in the outer mitochondrial membrane, was up-regulated after treatment with lycorine and was subsequently verified by western blot analysis. Further studies on mitochondria found that lycorine was able to increase abnormal mitochondria and increase mitochondrial membrane potential. Conclusions Lycorine can achieve the effect of resisting multiple myeloma by acting on VDAC2 and causing mitochondrial abnormalities.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-020-03053-2