Enhanced production of mono-rhamnolipid in Pseudomonas aeruginosa and application potential in agriculture and petroleum industry

•Rhamnolipid production was enhanced by 23.3% through knocking out rhlC gene.•Five kinds of mono-rhamnolipid were produced by the knockout strain SGΔrhlC.•Mono-rhamnolipid exhibited better antimicrobial activity to plant pathogens.•Rhamnolipid produced from SGΔrhlC showed greater emulsifying activit...

Full description

Saved in:
Bibliographic Details
Published in:Bioresource technology 2021-03, Vol.323, p.124605-124605, Article 124605
Main Authors: Zhao, Feng, Yuan, Menglin, Lei, Liying, Li, Chunyan, Xu, Xiaomeng
Format: Article
Language:English
Subjects:
Agriculture
Antimicrobial activity
Decanoates
Emulsifying activity
Glycolipids
Mono-rhamnolipids
Oil and Gas Industry
Oily sludge
Petroleum
Pseudomonas aeruginosa
Rhamnose - analogs & derivatives
Rhamnosyltransferase 2
Surface-Active Agents
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Rhamnolipid production was enhanced by 23.3% through knocking out rhlC gene.•Five kinds of mono-rhamnolipid were produced by the knockout strain SGΔrhlC.•Mono-rhamnolipid exhibited better antimicrobial activity to plant pathogens.•Rhamnolipid produced from SGΔrhlC showed greater emulsifying activity to crude oil.•This study is a step forward to tailor-made rhamnolipid biosynthesis and application. Differences in the rhamnolipid structures must result in its different activities, thus affecting its application effect. The rhlC gene in Pseudomonas aeruginosa SG was knocked out to construct strain P. aeruginosa SGΔrhlC. Rhamnolipid production was enhanced by 23.3% through knocking out rhlC gene. P. aeruginosa SGΔrhlC produced 14.22 g/L of rhamnolipid using glycerol and nitrate. Five kinds of mono-rhamnolipid but no di-rhamnolipid were produced by strain SGΔrhlC. The main rhamnolipid homologues were Rha-C10-C10, Rha-C10-C12:1 and Rha-C10-C12. Mono-rhamnolipid exhibited better antimicrobial activity to Escherichia coli, Staphylococcus aureus, Aspergillus niger and Penicillium chrysogenum. Rhamnolipid produced from strain SGΔrhlC showed greater emulsifying activity to crude oil with EI24 of 84.73%. Rhamnolipid produced from strain SGΔrhlC efficiently washed oily sludge at 35 °C. High-producing strain P. aeruginosa SGΔrhlC and its produced mono-rhamnolipid are more promising in agriculture and petroleum industry. This study is a step forward to the tailor-made biosynthesis and application of rhamnolipid.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2020.124605