Isolation and characterization of the novel Pseudomonas stutzeri bacteriophage 8P

Bacteriophage 8P was isolated with a Pseudomonas stutzeri strain isolated from an oil reservoir as its host bacterium. The phage genome comprises 63,753 base pairs with a G+C content of 64.35. The phage encodes 63 predicted proteins, and 27 of them were functionally assigned. No tRNA genes were foun...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2021-02, Vol.166 (2), p.601-606
Main Authors: Liu, Xinwu, Feng, Zhou, Fan, Xiangyu, Nie, Yong, Wu, Xiao-Lei
Format: Article
Language:English
Subjects:
Bacteriophages - genetics
Bacteriophages - isolation & purification
Base Composition - genetics
Biomedical and Life Sciences
Biomedicine
Brief Report
DNA, Viral - genetics
Genome, Viral - genetics
Genomes
Genomics - methods
Host Specificity - genetics
Infectious Diseases
Medical Microbiology
New species
Nucleotide sequence
Phages
Phylogeny
Podoviridae - genetics
Podoviridae - isolation & purification
Pseudomonas Phages - genetics
Pseudomonas Phages - isolation & purification
Pseudomonas stutzeri
Pseudomonas stutzeri - virology
Sequence Analysis, DNA - methods
Transmission electron microscopy
tRNA
Virology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bacteriophage 8P was isolated with a Pseudomonas stutzeri strain isolated from an oil reservoir as its host bacterium. The phage genome comprises 63,753 base pairs with a G+C content of 64.35. The phage encodes 63 predicted proteins, and 27 of them were functionally assigned. No tRNA genes were found. Comparative genomics analysis showed that 8P displayed some relatedness to F116-like phages (78% identity, 20% query coverage). The genome has very low sequence similarity to the other phage genomes in the GenBank database and Viral Sequence Database. Based on whole-genome analysis and transmission electron microscopy imaging, 8P is proposed to be a member of a new species in the genus Hollowayvirus , family Podoviridae .
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-020-04912-z