Overexpression of T-type calcium channel Cav3.1 in oral squamous cell carcinoma: association with proliferation and anti-apoptotic activity

Cav3.1, a subfamily of T-type calcium channel, is overexpressed in various human cancers and exerts important functions in tumor progression. This study is to identify the expression pattern and clinical significance of Cav3.1 in oral squamous cell carcinoma (OSCC). Firstly, the expression levels of...

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Bibliographic Details
Published in:Journal of molecular histology 2021-06, Vol.52 (3), p.511-520
Main Authors: Li, Rui-Fang, Man, Qi-Wen, Liu, Jin-Yuan, Zheng, Yue-Yu, Gao, Xin, Liu, Hai-Ming
Format: Article
Language:English
Subjects:
Apoptosis
Bcl-2 protein
Biomedical and Life Sciences
Biomedicine
Calcium
Calcium channels (T-type)
Calcium channels (voltage-gated)
Cell Biology
Cell proliferation
Developmental Biology
Dysplasia
Gene expression
Immunohistochemistry
Kinases
Life Sciences
mRNA
Mucosa
Oral cancer
Oral squamous cell carcinoma
Original Paper
Proliferating cell nuclear antigen
RNA-mediated interference
Squamous cell carcinoma
Statistical analysis
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Summary:Cav3.1, a subfamily of T-type calcium channel, is overexpressed in various human cancers and exerts important functions in tumor progression. This study is to identify the expression pattern and clinical significance of Cav3.1 in oral squamous cell carcinoma (OSCC). Firstly, the expression levels of Cav3.1 in oral mucosa (OM), dysplasia and oral squamous cell carcinoma (OSCC) were determined and compared by real-time quantitative PCR and Western blot analysis. After that, human tissue microarrays, containing 29 OM, 23 dysplasia and 122 primary OSCC samples, were applied to investigate the expression levels of Cav3.1, proliferation markers [Ki-67, proliferating cell nuclear antigen (PCNA)] and cellular anti-apoptosis markers [B cell lymphoma 2 (Bcl-2)] by immunohistochemistry and digital pathology analysis. In addition, we determined the function of Cav3.1 using knockdown assays of Cav3.1 in vitro. The results demonstrated that the mRNA and protein expression of Cav3.1 were significantly higher in OSCC specimens, and Cav3.1 expression in primary OSCCs was correlated with tumor size and pathological grade. Statistical analysis of immunohistochemical staining showed that Cav3.1 was closely correlated with Ki-67, PCNA and Bcl-2. Functional studies showed that the knockdown of Cav3.1 in OSCC cell lines using RNA interference influenced cell proliferation and apoptosis in vitro. Taken together, these findings suggested that Cav3.1 is overexpressed in OSCC tissues, also associated with proliferative and anti-apoptotic activity in oral squamous cell carcinoma.
ISSN:1567-2379
1567-2387
DOI:10.1007/s10735-020-09937-x