Differential DNA Methylation Profiles in Patients with Temporal Lobe Epilepsy and Hippocampal Sclerosis ILAE Type I

Hippocampal sclerosis (HS) is one of the most prevalent pathological types of temporal lobe epilepsy (TLE), and it significantly affects patient prognoses. The methylation of DNA plays an important role in the development of epilepsy. However, few studies have focused on HS subtypes to determine DNA...

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Bibliographic Details
Published in:Journal of molecular neuroscience 2021-09, Vol.71 (9), p.1951-1966
Main Authors: Zhang, Wang, Wang, Haiyang, Liu, Binchao, Jiang, Miaomiao, Gu, Yifei, Yan, Shi, Han, Xian, Hou, Alicia Y., Tang, Chongyang, Jiang, Zhenfeng, Shen, Hong, Na, Meng, Lin, Zhiguo
Format: Article
Language:English
Subjects:
Adolescent
Adult
Biomedical and Life Sciences
Biomedicine
Bisulfite
Cell Biology
Chromosomal Proteins, Non-Histone - genetics
Deoxyribonucleic acid
DNA
DNA Methylation
Epigenetics
Epigenome
Epilepsy
Epilepsy, Temporal Lobe - genetics
Epilepsy, Temporal Lobe - pathology
Female
Gene sequencing
Genes
Genomes
Hippocampus
Hippocampus - pathology
Humans
Male
Membrane Proteins - genetics
Neurochemistry
Neurology
Neurosciences
Pathogenesis
Patients
Proteomics
ras GTPase-Activating Proteins - genetics
Repressor Proteins - genetics
Sclerosis
Temporal lobe
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Summary:Hippocampal sclerosis (HS) is one of the most prevalent pathological types of temporal lobe epilepsy (TLE), and it significantly affects patient prognoses. The methylation of DNA plays an important role in the development of epilepsy. However, few studies have focused on HS subtypes to determine DNA methylation profiles in TLE. This study aimed to determine the pathogenesis of TLE from an epigenetic perspective in patients with TLE-HS type I (TLE-HSTI) and TLE without HS (TLE-nHS) using whole-genome bisulfite sequencing (WGBS). We defined 1171 hypermethylated and 2537 hypomethylated regions and found 632 differentially methylated genes (DMG) in the promoter region that were primarily involved in the regulation of various aspects of epilepsy development. Twelve DMG overlapped with differentially expressed genes (DEG) in the promoter region, and RT-qPCR findings revealed significant overexpression of the SBNO2 , CBX3 , RASAL3 , and TMBIM4 genes in TLE-HSTI. We present the first systematic analysis of methylation profiles of TLE-HSTI and TLE-nHS from an epigenetic perspective using WGBS. Overall, our preliminary data highlight the underlying mechanism of TLE-HSTI, providing a new perspective for guiding treatment of TLE.
ISSN:0895-8696
1559-1166
DOI:10.1007/s12031-020-01780-9