High-Spatial-Resolution Multimodal Imaging by Tapping-Mode Scanning Probe Electrospray Ionization with Feedback Control

Direct extraction and ionization techniques using minute amounts of solvent can be employed for the rapid analysis of chemical components in a sample without any sample preparation steps. This type of approach is important for mass spectrometry imaging of samples with multiple chemical components th...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-02, Vol.93 (4), p.2263-2272
Main Authors: Otsuka, Yoichi, Kamihoriuchi, Bui, Takeuchi, Aya, Iwata, Futoshi, Tortorella, Sara, Matsumoto, Takuya
Format: Article
Language:English
Subjects:
Amplitudes
Animal tissues
Chemistry
Control systems
Electrospraying
Feedback
Feedback control
Image resolution
Ionization
Ions
Mass spectrometry
Mass spectroscopy
Measurement techniques
Neuroimaging
Rhodamine
Sample preparation
Scanning
Solvents
Spatial discrimination
Spatial distribution
Spatial resolution
Thin films
Tissues
Vibration
Vibration measurement
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Summary:Direct extraction and ionization techniques using minute amounts of solvent can be employed for the rapid analysis of chemical components in a sample without any sample preparation steps. This type of approach is important for mass spectrometry imaging of samples with multiple chemical components that have different spatial distributions (i.e., biological tissues). To improve the spatial resolution of such imaging, it is necessary to reduce the solvent volume for extraction and deliver it to the sample surface. This report describes a feedback control system applied to tapping-mode scanning probe electrospray ionization. By combining the measurement technique of capillary probe vibration with the dynamic distance control system between the probe and the sample, the vibration amplitude of the probe is maintained while the probe scans over uneven samples. This method allows simultaneous high-resolution imaging of molecular distribution, surface topography, and amplitude/phase changes in the probe vibration. Such multimodal imaging is demonstrated on rhodamine B thin films in microwells and on a mouse brain tissue section. This technique can generally be applied to examine the multidimensional molecular distribution and the surface profiles of various objects.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c04144