Methanolic extract of Cariniana rubra Gardner ex Miers stem bark negatively regulate the leukocyte migration and TNF-α and up-regulate the annexin-A1 expression

• Published in: Journal of ethnopharmacology 2021-04, Vol.270, p.113778-113778, Article 113778
• Main Authors: Silva, Donata Norman Paulino Brandão, Adriana, Flach, Martins, Domingos Tabajara de Oliveira, Borges, Quessi Irias, Lindote, Marcus Vitor Nunes, Zoratti, Marco Túlio Ramalho, Oliveira, Ruberlei Godinho de, Torquato, Heron Fernandes Vieira, Gazoni, Vanessa Fátima, Costa, Luiz Antonio Mendonça Alves da, Souza, Edineide Cristina Alexandre de, Silva, Felipe Moura Araújo da, Arunachalam, Karuppusamy, Damazo, Amilcar Sabino
• Format: Article
• Language: English
• Subjects: Air pouch; Annexin A1; Cariniana rubra, anti-inflammatory; Gallic acid; Mice; Terpenes; TNF-α
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2021.113778

Cariniana rubra Gardner ex Miers (Lecythidaceae), is a native and endemic tree in Brazil, whose inner stem bark decoction preparation is used in folk medicine to treat various inflammatory disorders. Previous scientific reports confirmed its popular use as an anti-inflammatory, without, however, evaluating its action mechanisms. The objective of this study was to determine the cytotoxicity and anti-inflammatory mechanism of action of the methanolic extract of Cariniana rubra (MECr), using experimental models in vivo and in vitro, as well as to identify secondary metabolites present in the extract. The MECr was prepared by maceration of inner stem bark powder in methanol (1:10 w/v). The in vitro cytotoxicity effect was evaluated in CHO-k1 cells. The Hippocratic screening test was conducted to evaluate the acute toxicity of MECr in mice. The actions of MECr on leukocyte migration, cytokine levels (IL-1β and TNF-α) and annexin-A1 (AnxA1) expression, were carried out on lambda-type carrageenan air pouch inflammation model in Swiss mice. Additionally, the phytochemical analysis of MECr was carried out by thin-layer chromatography (TLC) and spectrometric mass analysis with electrospray ionization ESI(−)/MS and gas chromatography-mass spectrometry (GC-MS). Treatment of CHO-k1 cells for 24 h with MECr did not cause cytotoxicity (IC50 > 200 μg/mL), however, the MECr was shown to be cytotoxic after 72 h of cell exposure (IC50 = 19.90 ± 3.51 μg/mL). In the Hippocratic test, oral treatment of mice with 750, 1500, or 3000 mg/kg of MECr did not show any histopathological changes and mortality during the 14 days of observation. In the carrageenan air pouch inflammation model, MECr reduced (p