Purification and characterization of bromelain from pineapple (Ananas comosus L.) peel waste

• Published in: Journal of food science 2021-02, Vol.86 (2), p.385-393
• Main Authors: Zhou, Wei, Ye, Cuizhu, Geng, Lijing, Chen, Guannan, Wang, Xiaoyu, Chen, Weijie, Sa, Rina, Zhang, Junpeng, Zhang, Xiang
• Format: Article
• Language: English
• Subjects: Acetic acid; Aluminum; Ananas - enzymology; Antioxidants; Bromelains - antagonists & inhibitors; Bromelains - isolation & purification; Bromelains - metabolism; Butanol; Calcium; Calcium ions; Citric acid; Deactivation; Economics; Enzyme Inhibitors - pharmacology; enzyme kinetics; Enzyme Stability; Ethanol; Ethylenediamine; Food industry; Food processing; Food processing industry; Food production; Fruit - enzymology; Fruits; Hot Temperature; Inactivation; Iron; Kinetics; Maltose; pineapple proteinase; Pineapples; Potassium; Profitability; Purification; Sodium; Sodium citrate; Sodium nitrite; Stability tests; Sustainability
• ISSN: 0022-1147; 1750-3841
• DOI: 10.1111/1750-3841.15563

Bromelain is widely used in food industry and pharmaceutical products due to its strong antioxidant properties. Therefore, the extraction of bromelain from pineapple peel may improve the profitability and sustainability of pineapple industry. The aim of this work is to show the purification, stability, and kinetics of bromelain from pineapple peel. By studying the stability of purified bromelain (PB), we found that the activity of PB was inhibited by Fe3+, Al3+, methanol, ethanol, and n‐butyl alcohol, while it was increased in the presence of Ca2+, ethylenediamine tetra acetic acid, glucose, D‐xylose, maltose, potassium sodium tartrate, sodium citrate, citric acid, and sodium nitrite. These stability tests will expand the application and space acquisition of bromelain. The kinetics study indicated that the thermal inactivation of PB was conforming to the first‐order reaction and the half‐life (t1/2) of PB under different temperature conditions (45, 55, 65, and 75 °C) was 81.54, 31.12, 10.28, and 5.23 min, respectively. Therefore, the inactivation time of PB can be predicted at different temperatures for food heating processing. Practical Application The potential of utilizing pineapple peel for bromelain extraction might improve the profitability and sustainability of the pineapple industry.