N-terminal truncated RHT-1 proteins generated by translational reinitiation cause semi-dwarfing of wheat Green Revolution alleles

• Published in: Molecular plant 2021-04, Vol.14 (4), p.679-687
• Main Authors: Van De Velde, Karel, Thomas, Stephen G., Heyse, Floor, Kaspar, Rim, Van Der Straeten, Dominique, Rohde, Antje
• Format: Article
• Language: English
• Subjects: DELLA; gibberellin (GA) signaling; Green Revolution; Rht-1 dwarfing alleles; translational reinitiation; wheat
• ISSN: 1674-2052; 1752-9867
• DOI: 10.1016/j.molp.2021.01.002

The unprecedented wheat yield increases during the Green Revolution were achieved through the introduction of the Reduced height (Rht)-B1b and Rht-D1b semi-dwarfing alleles. These Rht-1 alleles encode growth-repressing DELLA genes containing a stop codon within their open reading frame that confers gibberellin (GA)-insensitive semi-dwarfism. In this study, we successfully took the hurdle of detecting wild-type RHT-1 proteins in different wheat organs and confirmed their degradation in response to GAs. We further demonstrated that Rht-B1b and Rht-D1b produce N-terminal truncated proteins through translational reinitiation. Expression of these N-terminal truncated proteins in transgenic lines and in Rht-D1c, an allele containing multiple Rht-D1b copies, demonstrated their ability to cause strong dwarfism, resulting from their insensitivity to GA-mediated degradation. N-terminal truncated proteins were detected in spikes and nodes, but not in the aleurone layers. Since Rht-B1b and Rht-D1b alleles cause dwarfism but have wild-type dormancy, this finding suggests that tissue-specific differences in translational reinitiation may explain why the Rht-1 alleles reduce plant height without affecting dormancy. Taken together, our findings not only reveal the molecular mechanism underlying the Green Revolution but also demonstrate that translational reinitiation in the main open reading frame occurs in plants. The wheat yield increases during the Green Revolution were achieved through the introduction of the Rht-1 alleles (DELLA alleles) that cause dwarfism, without affecting dormancy. This study reveals that the Green Revolution alleles produce N-terminal truncated proteins through translational reinitiation in spikes and nodes, but not in the aleurone layers of grains.