NEase-based amplification for detection of miRNA, multiple miRNAs and circRNA

MiRNAs are particularly desirable biomarkers for the diagnosis of many cancers, but the methods for simultaneous detection of multiple miRNAs are rarely published. In addition, circRNAs are a novel class of non-coding RNAs and it has been accepted that circRNAs participate in regulating various biol...

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Bibliographic Details
Published in:Analytica chimica acta 2021-02, Vol.1145, p.52-58
Main Authors: Zhang, Yajun, Zhang, Qiuyang, Weng, Xiaocheng, Du, Yuhao, Zhou, Xiang
Format: Article
Language:English
Subjects:
Biomarkers
CircRNA
Humans
MicroRNAs - genetics
MiRNA
Neoplasms
RNA, Circular
Simultaneous detection
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Summary:MiRNAs are particularly desirable biomarkers for the diagnosis of many cancers, but the methods for simultaneous detection of multiple miRNAs are rarely published. In addition, circRNAs are a novel class of non-coding RNAs and it has been accepted that circRNAs participate in regulating various biological processes. Herein, we design a method using NEase which has highly selectivity and activity to amplify fluorescence signal, so single miRNA, circRNA and multiple miRNAs could be simply quantified through our proposed assay. Furthermore, this method also can detect miRNA level in different cell lines. [Display omitted] •We design a method using NEase which has highly selectivity and activity to amplify fluorescence signal.•Single miRNA, circRNA and multiple miRNAs could be simultaneously quantified through our proposed assay.•This method could also detect miRNA in total RNA extracted from different cell lines.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2020.12.024