In‐depth proteome of perilymph in guinea pig model

The vast majority of sensorineural hearing loss is caused by impairment of the inner ear cells. Proteomic analysis of perilymph may therefore improve our understanding of inner ear diseases and hearing loss. However, the investigation of the human perilymph proteome was limited due to technical diff...

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Published in:Proteomics (Weinheim) 2021-03, Vol.21 (5), p.e2000138-n/a
Main Authors: Lee, Jung Hun, Hwang, Yu‐Jung, Li, Hui, Kim, Hyeyoon, Suh, Myung‐Whan, Han, Dohyun, Oh, Seung‐Ha
Format: Article
Language:English
Subjects:
Ear
guinea pig
Guinea pigs
Hearing
Hearing loss
Hearing protection
Inner ear
perilymph
proteome
Proteomes
Swine
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creator Lee, Jung Hun
Hwang, Yu‐Jung
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description The vast majority of sensorineural hearing loss is caused by impairment of the inner ear cells. Proteomic analysis of perilymph may therefore improve our understanding of inner ear diseases and hearing loss. However, the investigation of the human perilymph proteome was limited due to technical difficulties in perilymph sampling. The guinea pig (Cavia porcellus) is frequently used as an experimental model in preclinical hearing research. In this study, we analyzed samples of perilymph collected from 12 guinea pigs to overcome limited experimental information regarding its proteome. We identified a total of 1413 proteins, establishing a greatly expanded proteome of the previously inferred guinea pig perilymph. This provides a comprehensive proteomic resource for the research community, which will facilitate future molecular‐phenotypic studies using the guinea pig as an experimental model of relevance to human inner ear biology.
doi_str_mv 10.1002/pmic.202000138
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subjects Ear
guinea pig
Guinea pigs
Hearing
Hearing loss
Hearing protection
Inner ear
perilymph
proteome
Proteomes
Swine
title In‐depth proteome of perilymph in guinea pig model
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