Fed-batch production of Thermothelomyces thermophilus lignin peroxidase using a recombinant Aspergillus nidulans strain in stirred-tank bioreactor

•A novel Thermothelomyces thermophilus lignin peroxidase (LiP) was produced.•Scale-up production of LiP was conducted in stirred-tank bioreactor at 1.5 L scale.•Different fed-batch strategies (continuous, multi-pulse, and DO-stat) were studied.•Maximum LiP activity of 1,645 mU/L achieved via multi-p...

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Bibliographic Details
Published in:Bioresource technology 2021-04, Vol.325, p.124700-124700, Article 124700
Main Authors: Liu, Enshi, Segato, Fernando, Wilkins, Mark R.
Format: Article
Language:English
Subjects:
Aspergillus nidulans - genetics
Bioreactors
Fed-batch fermentation
Fungal enzyme production
Heterologous protein expression
Lignin
Lignin-degrading enzymes
Peroxidases - genetics
Process optimization
Sordariales
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Summary:•A novel Thermothelomyces thermophilus lignin peroxidase (LiP) was produced.•Scale-up production of LiP was conducted in stirred-tank bioreactor at 1.5 L scale.•Different fed-batch strategies (continuous, multi-pulse, and DO-stat) were studied.•Maximum LiP activity of 1,645 mU/L achieved via multi-pulse fed-batch production.•Tangential flow ultrafiltration was effective at concentrating crude LiP. Enzymatic lignin depolymerization is considered a favorable approach to utilize lignin due to the higher selectivity and less energy requirement when compared to thermochemical lignin valorization. Lignin peroxidase (LiP) is one of the key enzymes involved in lignin degradation and possesses high redox potential to oxidize non-phenolic structures and phenolic compounds in lignin. However, the production of LiP is mainly from white-rot fungi at small scales. It is critical to discover new LiP from other microorganisms and produce LiP at large scales. This study aims to produce a novel LiP originally from Thermothelomyces thermophiles using a recombinant Aspergillus nidulans strain. The LiP production medium was optimized, and different fed-batch strategies for LiP production were investigated to improve LiP activity, yield, and productivity. Results demonstrated that LiP production was enhanced by using multi-pulse fed-batch fermentation. A maximum LiP activity of 1,645 mU/L with a protein concentration of 0.26 g/L was achieved.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2021.124700