Merozoite-Protein Loaded Liposomes Protect against Challenge in Two Murine Models of Plasmodium Infection

Liposomes loaded with GPI-anchored proteins induce a strong and effective response against protozoan pathogens when immunized in mice. On the basis of results using Plasmodium falciparum merozoite proteins loaded on liposomes, we tested whether a similar approach would be protective in the P. yoelii...

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Bibliographic Details
Published in:ACS biomaterials science & engineering 2016-12, Vol.2 (12), p.2276-2286
Main Authors: Fotoran, Wesley L, Colhone, Marcelle C, Ciancaglini, Pietro, Stabeli, Rodrigo G, Wunderlich, Gerhard
Format: Article
Language:English
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Summary:Liposomes loaded with GPI-anchored proteins induce a strong and effective response against protozoan pathogens when immunized in mice. On the basis of results using Plasmodium falciparum merozoite proteins loaded on liposomes, we tested whether a similar approach would be protective in the P. yoelii XL and P. berghei NK65 models of lethal murine malaria infections. We also analyzed the enhancing capacity of additional adjuvants. As expected, merozoite protein loaded liposomes provided strong humoral responses (IgG titers of 105 against MSP119) in BALB/c and C57BL/6 host backgrounds and showed total protection in lethal challenges (100% survival) with P. yoelii, while protection against lethal challenge with P. berghei NK was at least partial (11%–20% in C57BL/6 mice and no protection in BALB/c). Thus, immunization with proteoliposomes is not only highly immunogenic but also provides a protective response in widely used murine models of malaria.
ISSN:2373-9878
2373-9878
DOI:10.1021/acsbiomaterials.6b00492