Liposome Enhanced Detection of Amyloid Protein Aggregates

Thioflavin-T is used to image amyloid aggregates because of the excellent turn-on fluorescence properties, but binding affinities are low. By mounting multiple dye units on the surface of a vesicle, the binding affinity for α-synuclein fibrils is increased by three orders of magnitude, and the optic...

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Bibliographic Details
Published in:Organic letters 2021-02, Vol.23 (3), p.647-650
Main Authors: Kocsis, Istvan, Sanna, Elena, Hunter, Christopher A
Format: Article
Language:English
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Summary:Thioflavin-T is used to image amyloid aggregates because of the excellent turn-on fluorescence properties, but binding affinities are low. By mounting multiple dye units on the surface of a vesicle, the binding affinity for α-synuclein fibrils is increased by three orders of magnitude, and the optical response is increased. Cooperative interactions of the dye headgroup and lipid with the protein provide a general strategy for the construction of multivalent amyloid probes based on vesicles.
ISSN:1523-7060
1523-7052
DOI:10.1021/acs.orglett.0c03597