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Stable, fertile lines produced by hybridization between allotetraploids Brassica juncea (AABB) and Brassica carinata (BBCC) have merged the A and C genomes
Summary Many flowering plant taxa contain allopolyploids that share one or more genomes in common. In the Brassica genus, crop species Brassica juncea and Brassica carinata share the B genome, with 2n = AABB and 2n = BBCC genome complements, respectively. Hybridization results in 2n = BBAC hybrids,...
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| Published in: | The New phytologist 2021-05, Vol.230 (3), p.1242-1257 |
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| Main Authors: | , , , , , , , |
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| container_end_page | 1257 |
| container_issue | 3 |
| container_start_page | 1242 |
| container_title | The New phytologist |
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| creator | Katche, Elvis Gaebelein, Roman Idris, Zurianti Vasquez‐Teuber, Paula Lo, Yu‐tzu Nugent, David Batley, Jacqueline Mason, Annaliese S. |
| description | Summary
Many flowering plant taxa contain allopolyploids that share one or more genomes in common. In the Brassica genus, crop species Brassica juncea and Brassica carinata share the B genome, with 2n = AABB and 2n = BBCC genome complements, respectively. Hybridization results in 2n = BBAC hybrids, but the fate of these hybrids over generations of self‐pollination has never been reported.
We produced and characterized B. juncea × B. carinata (2n = BBAC) interspecific hybrids over six generations of self‐pollination under selection for high fertility using a combination of genotyping, fertility phenotyping, and cytogenetics techniques.
Meiotic pairing behaviour improved from 68% bivalents in the F1 to 98% in the S5/S6 generations, and initially low hybrid fertility also increased to parent species levels. The S5/S6 hybrids contained an intact B genome (16 chromosomes) plus a new, stable A/C genome (18–20 chromosomes) resulting from recombination and restructuring of A and C‐genome chromosomes.
Our results provide the first experimental evidence that two genomes can come together to form a new, restructured genome in hybridization events between two allotetraploid species that share a common genome. This mechanism should be considered in interpreting phylogenies in taxa with multiple allopolyploid species. |
| doi_str_mv | 10.1111/nph.17225 |
| format | article |
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Many flowering plant taxa contain allopolyploids that share one or more genomes in common. In the Brassica genus, crop species Brassica juncea and Brassica carinata share the B genome, with 2n = AABB and 2n = BBCC genome complements, respectively. Hybridization results in 2n = BBAC hybrids, but the fate of these hybrids over generations of self‐pollination has never been reported.
We produced and characterized B. juncea × B. carinata (2n = BBAC) interspecific hybrids over six generations of self‐pollination under selection for high fertility using a combination of genotyping, fertility phenotyping, and cytogenetics techniques.
Meiotic pairing behaviour improved from 68% bivalents in the F1 to 98% in the S5/S6 generations, and initially low hybrid fertility also increased to parent species levels. The S5/S6 hybrids contained an intact B genome (16 chromosomes) plus a new, stable A/C genome (18–20 chromosomes) resulting from recombination and restructuring of A and C‐genome chromosomes.
Our results provide the first experimental evidence that two genomes can come together to form a new, restructured genome in hybridization events between two allotetraploid species that share a common genome. This mechanism should be considered in interpreting phylogenies in taxa with multiple allopolyploid species.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.17225</identifier><identifier>PMID: 33476056</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Brassica ; Brassica carinata ; Brassica juncea ; Chromosomes ; Chromosomes, Plant - genetics ; Cytogenetics ; Fertility ; Fertility - genetics ; Flowering ; genome rearrangement ; Genome, Plant - genetics ; Genomes ; Genotyping ; homoeologous exchanges ; Hybridization ; Hybridization, Genetic ; Hybrids ; Interspecific ; interspecific hybridization ; Meiosis ; Mustard Plant - genetics ; Phenotyping ; Plant reproduction ; Pollination ; Polyploidy ; Recombination ; Species ; Taxa</subject><ispartof>The New phytologist, 2021-05, Vol.230 (3), p.1242-1257</ispartof><rights>2021 The Authors © 2021 New Phytologist Trust</rights><rights>2021 The Authors New Phytologist © 2021 New Phytologist Trust.</rights><rights>2021. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3535-70db1f2c5e3e461a17334383823b13ccb164e7f45d6d9085d58d4a16a55a09583</citedby><cites>FETCH-LOGICAL-c3535-70db1f2c5e3e461a17334383823b13ccb164e7f45d6d9085d58d4a16a55a09583</cites><orcidid>0000-0002-5391-5824 ; 0000-0003-2701-7964 ; 0000-0002-3070-5656</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33476056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Katche, Elvis</creatorcontrib><creatorcontrib>Gaebelein, Roman</creatorcontrib><creatorcontrib>Idris, Zurianti</creatorcontrib><creatorcontrib>Vasquez‐Teuber, Paula</creatorcontrib><creatorcontrib>Lo, Yu‐tzu</creatorcontrib><creatorcontrib>Nugent, David</creatorcontrib><creatorcontrib>Batley, Jacqueline</creatorcontrib><creatorcontrib>Mason, Annaliese S.</creatorcontrib><title>Stable, fertile lines produced by hybridization between allotetraploids Brassica juncea (AABB) and Brassica carinata (BBCC) have merged the A and C genomes</title><title>The New phytologist</title><addtitle>New Phytol</addtitle><description>Summary
Many flowering plant taxa contain allopolyploids that share one or more genomes in common. In the Brassica genus, crop species Brassica juncea and Brassica carinata share the B genome, with 2n = AABB and 2n = BBCC genome complements, respectively. Hybridization results in 2n = BBAC hybrids, but the fate of these hybrids over generations of self‐pollination has never been reported.
We produced and characterized B. juncea × B. carinata (2n = BBAC) interspecific hybrids over six generations of self‐pollination under selection for high fertility using a combination of genotyping, fertility phenotyping, and cytogenetics techniques.
Meiotic pairing behaviour improved from 68% bivalents in the F1 to 98% in the S5/S6 generations, and initially low hybrid fertility also increased to parent species levels. The S5/S6 hybrids contained an intact B genome (16 chromosomes) plus a new, stable A/C genome (18–20 chromosomes) resulting from recombination and restructuring of A and C‐genome chromosomes.
Our results provide the first experimental evidence that two genomes can come together to form a new, restructured genome in hybridization events between two allotetraploid species that share a common genome. This mechanism should be considered in interpreting phylogenies in taxa with multiple allopolyploid species.</description><subject>Brassica</subject><subject>Brassica carinata</subject><subject>Brassica juncea</subject><subject>Chromosomes</subject><subject>Chromosomes, Plant - genetics</subject><subject>Cytogenetics</subject><subject>Fertility</subject><subject>Fertility - genetics</subject><subject>Flowering</subject><subject>genome rearrangement</subject><subject>Genome, Plant - genetics</subject><subject>Genomes</subject><subject>Genotyping</subject><subject>homoeologous exchanges</subject><subject>Hybridization</subject><subject>Hybridization, Genetic</subject><subject>Hybrids</subject><subject>Interspecific</subject><subject>interspecific hybridization</subject><subject>Meiosis</subject><subject>Mustard Plant - genetics</subject><subject>Phenotyping</subject><subject>Plant reproduction</subject><subject>Pollination</subject><subject>Polyploidy</subject><subject>Recombination</subject><subject>Species</subject><subject>Taxa</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><recordid>eNp1kc9u1DAQhy0EokvhwAsgS1y6Emn9J3aS4yYCilQBEiBxsxx70vUqcba2Q7W8Ci-L6RaQkPBlDvPpN575EHpOyTnN78Lvt-e0Ykw8QCtayqaoKa8eohUhrC5kKb-eoCcx7gghjZDsMTrhvKwkEXKFfnxKuh_hFR4gJDcCHp2HiPdhtosBi_sD3h764Kz7rpObPe4h3QJ4rMdxTpCC3o-zsxG3QcfojMa7xRvQ-Gyzads11t7-bRkdnNcpN9u269Z4q78BniBc50FpC3hzh3f4Gvw8QXyKHg16jPDsvp6iL29ef-4ui6sPb991m6vCcMFFURHb04EZARxKSTWt8nq85jXjPeXG9FSWUA2lsNI2pBZW1LbUVGohdD5IzU_R2TE3b32zQExqctHAOGoP8xIVK6umIk3DeEZf_oPu5iX4_DvFBGkY5axkmVofKRPmGAMMah_cpMNBUaJ-GVPZmLozltkX94lLP4H9Q_5WlIGLI3Cb9Rz-n6Tef7w8Rv4EeCCeYA</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Katche, Elvis</creator><creator>Gaebelein, Roman</creator><creator>Idris, Zurianti</creator><creator>Vasquez‐Teuber, Paula</creator><creator>Lo, Yu‐tzu</creator><creator>Nugent, David</creator><creator>Batley, Jacqueline</creator><creator>Mason, Annaliese S.</creator><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5391-5824</orcidid><orcidid>https://orcid.org/0000-0003-2701-7964</orcidid><orcidid>https://orcid.org/0000-0002-3070-5656</orcidid></search><sort><creationdate>202105</creationdate><title>Stable, fertile lines produced by hybridization between allotetraploids Brassica juncea (AABB) and Brassica carinata (BBCC) have merged the A and C genomes</title><author>Katche, Elvis ; Gaebelein, Roman ; Idris, Zurianti ; Vasquez‐Teuber, Paula ; Lo, Yu‐tzu ; Nugent, David ; Batley, Jacqueline ; Mason, Annaliese S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3535-70db1f2c5e3e461a17334383823b13ccb164e7f45d6d9085d58d4a16a55a09583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Brassica</topic><topic>Brassica carinata</topic><topic>Brassica juncea</topic><topic>Chromosomes</topic><topic>Chromosomes, Plant - genetics</topic><topic>Cytogenetics</topic><topic>Fertility</topic><topic>Fertility - genetics</topic><topic>Flowering</topic><topic>genome rearrangement</topic><topic>Genome, Plant - genetics</topic><topic>Genomes</topic><topic>Genotyping</topic><topic>homoeologous exchanges</topic><topic>Hybridization</topic><topic>Hybridization, Genetic</topic><topic>Hybrids</topic><topic>Interspecific</topic><topic>interspecific hybridization</topic><topic>Meiosis</topic><topic>Mustard Plant - genetics</topic><topic>Phenotyping</topic><topic>Plant reproduction</topic><topic>Pollination</topic><topic>Polyploidy</topic><topic>Recombination</topic><topic>Species</topic><topic>Taxa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Katche, Elvis</creatorcontrib><creatorcontrib>Gaebelein, Roman</creatorcontrib><creatorcontrib>Idris, Zurianti</creatorcontrib><creatorcontrib>Vasquez‐Teuber, Paula</creatorcontrib><creatorcontrib>Lo, Yu‐tzu</creatorcontrib><creatorcontrib>Nugent, David</creatorcontrib><creatorcontrib>Batley, Jacqueline</creatorcontrib><creatorcontrib>Mason, Annaliese S.</creatorcontrib><collection>Open Access: Wiley-Blackwell Open Access Journals</collection><collection>Wiley Online Library Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Katche, Elvis</au><au>Gaebelein, Roman</au><au>Idris, Zurianti</au><au>Vasquez‐Teuber, Paula</au><au>Lo, Yu‐tzu</au><au>Nugent, David</au><au>Batley, Jacqueline</au><au>Mason, Annaliese S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stable, fertile lines produced by hybridization between allotetraploids Brassica juncea (AABB) and Brassica carinata (BBCC) have merged the A and C genomes</atitle><jtitle>The New phytologist</jtitle><addtitle>New Phytol</addtitle><date>2021-05</date><risdate>2021</risdate><volume>230</volume><issue>3</issue><spage>1242</spage><epage>1257</epage><pages>1242-1257</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>Summary
Many flowering plant taxa contain allopolyploids that share one or more genomes in common. In the Brassica genus, crop species Brassica juncea and Brassica carinata share the B genome, with 2n = AABB and 2n = BBCC genome complements, respectively. Hybridization results in 2n = BBAC hybrids, but the fate of these hybrids over generations of self‐pollination has never been reported.
We produced and characterized B. juncea × B. carinata (2n = BBAC) interspecific hybrids over six generations of self‐pollination under selection for high fertility using a combination of genotyping, fertility phenotyping, and cytogenetics techniques.
Meiotic pairing behaviour improved from 68% bivalents in the F1 to 98% in the S5/S6 generations, and initially low hybrid fertility also increased to parent species levels. The S5/S6 hybrids contained an intact B genome (16 chromosomes) plus a new, stable A/C genome (18–20 chromosomes) resulting from recombination and restructuring of A and C‐genome chromosomes.
Our results provide the first experimental evidence that two genomes can come together to form a new, restructured genome in hybridization events between two allotetraploid species that share a common genome. This mechanism should be considered in interpreting phylogenies in taxa with multiple allopolyploid species.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33476056</pmid><doi>10.1111/nph.17225</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-5391-5824</orcidid><orcidid>https://orcid.org/0000-0003-2701-7964</orcidid><orcidid>https://orcid.org/0000-0002-3070-5656</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Brassica Brassica carinata Brassica juncea Chromosomes Chromosomes, Plant - genetics Cytogenetics Fertility Fertility - genetics Flowering genome rearrangement Genome, Plant - genetics Genomes Genotyping homoeologous exchanges Hybridization Hybridization, Genetic Hybrids Interspecific interspecific hybridization Meiosis Mustard Plant - genetics Phenotyping Plant reproduction Pollination Polyploidy Recombination Species Taxa |
| title | Stable, fertile lines produced by hybridization between allotetraploids Brassica juncea (AABB) and Brassica carinata (BBCC) have merged the A and C genomes |
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