Effects of calcium-activated potassium channel modulators on afterhyperpolarizing potentials in identified motor and mechanosensory neurons of the medicinal leech

Calcium-activated potassium (K Ca ) channels contribute to multiple neuronal properties including spike frequency and afterhyperpolarizing potentials (AHPs). K Ca channels are classified as K Ca 1.1, K Ca 2, or K Ca 3.1 based on single-channel conductance and pharmacology. Ca 2+ -dependent AHPs in v...

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Bibliographic Details
Published in:Journal of Comparative Physiology 2021, Vol.207 (1), p.69-85
Main Authors: Angstadt, James D., Rebel, Matthew I., Connolly, Megan K.
Format: Article
Language:English
Subjects:
1-Naphthylamine - analogs & derivatives
1-Naphthylamine - pharmacology
Animal Physiology
Animals
Biomedical and Life Sciences
Calcium
Calcium - metabolism
Calcium channels
Calcium conductance
Calcium ions
Channels
Firing pattern
Hirudo medicinalis - drug effects
Hirudo medicinalis - physiology
Life Sciences
Membrane Potentials
Modulators
Motor Neurons - drug effects
Motor Neurons - physiology
Neuromodulation
Neurons
Neurosciences
Original Paper
Pharmacology
Potassium
Potassium channels
Potassium channels (calcium-gated)
Potassium Channels, Calcium-Activated - metabolism
Pyrazoles - pharmacology
Pyrimidines - pharmacology
Resistance
Vertebrates
Zoology
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Summary:Calcium-activated potassium (K Ca ) channels contribute to multiple neuronal properties including spike frequency and afterhyperpolarizing potentials (AHPs). K Ca channels are classified as K Ca 1.1, K Ca 2, or K Ca 3.1 based on single-channel conductance and pharmacology. Ca 2+ -dependent AHPs in vertebrates are categorized as fast, medium, or slow. Fast and medium AHPs are generated by K Ca 1.1 and K Ca 2 channels, respectively. The K Ca subtype responsible for slow AHPs is unclear. Prolonged, Ca 2+ -dependent AHPs have been described in several leech neurons. Unfortunately, apamin and other K Ca blockers often prove ineffective in the leech. An alternative approach is to utilize K Ca modulators, which alter channel sensitivity to Ca 2+ . Vertebrate K Ca 2 channels are targeted selectively by the positive modulator CyPPA and the negative modulator NS8593. Here we show that AHPs in identified motor and mechanosensory leech neurons are enhanced by CyPPA and suppressed by NS8593. Our results indicate that K Ca 2 channels underlie prolonged AHPs in these neurons and suggest that K Ca 2 modulators may serve as effective tools to explore the role of K Ca channels in leech physiology.
ISSN:0340-7594
1432-1351
DOI:10.1007/s00359-021-01462-w