α-Tocopherol Acetate Attenuates Mitochondrial Oxygen Consumption and Maintains Primitive Cells within Mesenchymal Stromal Cell Population

• Published in: Stem cell reviews and reports 2021-08, Vol.17 (4), p.1390-1405
• Main Authors: Loncaric, Darija, Rodriguez, Laura, Debeissat, Christelle, Touya, Nicolas, Labat, Veronique, Villacreces, Arnaud, Bouzier-Sore, Anne-Karine, Pasquet, Jean-Max, Brunet de la Grange, Philippe, Vlaski-Lafarge, Marija, Pavlovic, Sonja, Ivanovic, Zoran
• Format: Article
• Language: English
• Subjects: Acetic acid; alpha-Tocopherol - pharmacology; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Cell Biology; Cell Differentiation; Electron transport; Gene expression; Glycolysis; Humans; Hypoxia-inducible factor 1a; Life Sciences; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; Mesenchyme; Mitochondria; Mitochondria - metabolism; Oxygen - metabolism; Oxygen consumption; Regenerative Medicine/Tissue Engineering; Stem Cells; Vitamin E
• ISSN: 2629-3269; 2629-3277
• DOI: 10.1007/s12015-020-10111-9

We present here the data showing, in standard cultures exposed to atmospheric O 2 concentration, that alpha-tocopherol acetate (α-TOA) has a positive impact on primitive cells inside mesenchymal stromal cell (MstroC) population, by maintaining their proliferative capacity. α-TOA decreases the O 2 consumption rate of MStroC probably by impacting respiratory chain complex II activity. This action, however, is not associated with a compensatory increase in glycolysis activity, in spite of the fact that the degradation of HIF-1α was decreased in presence of α-TOA. This is in line with a moderate enhancement of mtROS upon α-TOA treatment. However, the absence of glycolysis stimulation implies the inactivity of HIF-1α which might – if it were active – be related to the maintenance of stemness. It should be stressed that α-TOA might act directly on the gene expression as well as the mtROS themselves, which remains to be elucidated. Graphical abstract Alpha-tocopherol acetate (α-TOA), a synthetic vitamin E ester, attenuates electron flow through electron transport chain (ETC) which is probably associated with a moderate increase in mtROS in Mesenchymal Stromal Cells. α-TOA action results in enhancement of the proliferative capacity and maintenance of the differentiation potential of the mesenchymal stem and progenitor cells.